論文 - 詳細
| RRC ID | 44815 |
|---|---|
| 著者 | Sugiyama Y, Hagiya Y, Nakajima M, Ishizuka M, Tanaka T, Ogura S. |
| タイトル | The heme precursor 5-aminolevulinic acid disrupts the Warburg effect in tumor cells and induces caspase-dependent apoptosis. |
| ジャーナル | Oncol Rep |
| Abstract |
Our previous study demonstrated that 5-aminolevulinic acid (ALA) administered to mice stimulates oxidative phosphorylation by upregulation of the mitochondrial respiratory chain complex IV enzyme cytochrome c oxidase (COX). The present study investigated whether ALA disrupts the Warburg effect, which represents a shift in ATP generation from oxidative phosphorylation to glycolysis, protecting tumor cells against oxidative stress-mediated apoptosis. The human lung carcinoma cell line A549 exposed to ALA exhibited enhanced oxidative phosphorylation, which was indicated by an increase in COX protein expression and oxygen consumption. Furthermore, ALA suppressed glycolysis-mediated acidosis. This normalization of the ATP metabolic pathways significantly increased the generation of superoxide anion radical (O2•-) and the functional expression of active caspase-3, leading to caspase-dependent apoptosis. These data demonstrate that ALA inhibits the Warburg effect and induces cancer cell death. Use of this endogenous compound might constitute a novel approach to cancer therapy. |
| 巻・号 | 31(3) |
| ページ | 1282-6 |
| 公開日 | 2014-3-1 |
| DOI | 10.3892/or.2013.2945 |
| PMID | 24366173 |
| MeSH | Aminolevulinic Acid / pharmacology* Antineoplastic Agents / pharmacology* Apoptosis / drug effects* Caspase 3 / metabolism* Cell Line, Tumor Cell Survival / drug effects Drug Screening Assays, Antitumor Glycolysis / drug effects* Humans Hydrogen-Ion Concentration Oxidative Phosphorylation / drug effects Superoxides / metabolism |
| IF | 3.417 |
| 引用数 | 8 |
| WOS 分野 | ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | A549 |