| RRC ID |
44819
|
| 著者 |
Ninomiya K, Yamada R, Meisaku H, Shimizu N.
|
| タイトル |
Effect of ultrasound irradiation on bacterial internalization and bacteria-mediated gene transfer to cancer cells.
|
| ジャーナル |
Ultrason Sonochem
|
| Abstract |
The present study demonstrates that ultrasound irradiation can facilitate bacteria-mediated gene delivery (bactofection). Escherichia coli modified with avidin were employed as a vehicle for delivery of the green fluorescent protein (GFP) gene, a model heterologous gene, into the breast cancer cell line MCF-7. Avidin-mediated binding of E. coli to MCF-7 cells enhanced the internalization of E. coli by approximately 17%, irrespective of the use of ultrasound irradiation. Furthermore, the use of ultrasound irradiation increased the internalization by approximately 5%, irrespective of the presence of avidin on the E. coli cell surface. The percentages of GFP-expressing MCF-7 cells at 24h after bactofection were below 0.5% and 2% for the case with only avidin-modification of E. coli cell surface and only ultrasound irradiation, respectively. However, combining avidin modification with the ultrasound treatment increased this value to 8%. Thus, the use of avidin-modified bacteria in conjunction with ultrasound irradiation has potential as an effective strategy for tumor-targeted bactofection.
|
| 巻・号 |
21(3)
|
| ページ |
1187-93
|
| 公開日 |
2014-5-1
|
| DOI |
10.1016/j.ultsonch.2013.12.005
|
| PII |
S1350-4177(13)00311-8
|
| PMID |
24373691
|
| MeSH |
Avidin / metabolism
Cell Line, Tumor
Escherichia coli / genetics*
Escherichia coli / metabolism*
Green Fluorescent Proteins / genetics
Humans
MCF-7 Cells
Plasmids / genetics
Transfection / methods*
Ultrasonics*
|
| IF |
6.513
|
| 引用数 |
7
|
|
WOS 分野
|
ACOUSTICS
CHEMISTRY, MULTIDISCIPLINARY
|
| リソース情報 |
| ヒト・動物細胞 |
|
