| RRC ID |
44828
|
| Author |
Yazaki Y, Oyane A, Tsurushima H, Araki H, Sogo Y, Ito A, Yamazaki A.
|
| Title |
Coprecipitation of DNA-lipid complexes with apatite and comparison with superficial adsorption for gene transfer applications.
|
| Journal |
J Biomater Appl
|
| Abstract |
Apatite can mediate gene transfer into cells by serving as a safe and biocompatible immobilization matrix for DNA and transfection reagents. Recently, an apatite layer that immobilized DNA-lipid complexes was prepared by a coprecipitation process in a supersaturated calcium phosphate solution. This composite layer (DNA-lipid-apatite layer) showed a higher gene transfer capability than an apatite layer with superficially adsorbed DNA-lipid complexes (DNA-lipid-adsorbed apatite layer). In this study, the DNA-lipid-apatite layer and the DNA-lipid-adsorbed apatite layer were compared for their physicochemical properties and gene transfer capabilities. The higher gene transfer capability of the DNA-lipid-apatite layer compared with that of the DNA-lipid-adsorbed apatite layer was reconfirmed by a luciferase assay using epithelial-like CHO-K1 cells. Physicochemical structure analyses showed that the DNA-lipid-apatite layer possessed a larger capacity for DNA-lipid complexes than the DNA-lipid-adsorbed apatite layer. The DNA-lipid-apatite layer released DNA-lipid complexes in a slow and sustained manner, whereas the DNA-lipid-adsorbed apatite layer released them in short bursts. Consequently, the release of DNA-lipid complexes from the DNA-lipid-apatite layer was larger in amount and longer in duration than release from the DNA-lipid-adsorbed apatite layer. This difference in release profiles may be responsible for the higher gene transfer capability of the DNA-lipid-apatite layer compared with that of the DNA-lipid-adsorbed apatite layer. The coprecipitation process and the resulting DNA-lipid-apatite layer have many applications in tissue engineering.
|
| Volume |
28(6)
|
| Pages |
937-45
|
| Published |
2014-2-1
|
| DOI |
10.1177/0885328213486706
|
| PII |
28/6/937
|
| PMID |
24381202
|
| MeSH |
Adsorption
Animals
Apatites / chemistry*
CHO Cells
Cricetinae
Cricetulus
DNA / chemistry*
Gene Transfer Techniques*
Lipids / chemistry*
Microscopy, Electron, Scanning
X-Ray Diffraction
|
| IF |
2.22
|
| Times Cited |
5
|
|
WOS Category
|
ENGINEERING, BIOMEDICAL
MATERIALS SCIENCE, BIOMATERIALS
|
| Resource |
| Human and Animal Cells |
CHO-K1 |
