Reference - Detail
| RRC ID | 44832 |
|---|---|
| Author | Tsuji S, Yamashita M, Kageyama T, Ohtsu T, Suzuki K, Kato S, Akitomi J, Furuichi M, Waga I. |
| Title | Hishot display--a new combinatorial display for obtaining target-recognizing peptides. |
| Journal | PLoS One |
| Abstract |
Display technologies are procedures used for isolating target-recognizing peptides without using immunized animals. In this study, we describe a new display method, named Hishot display, that uses Escherichia coli and an expression plasmid to isolate target-recognizing peptides. This display method is based on the formation, in bacteria, of complexes between a polyhistidine (His)-tagged peptide including random sequences and the peptide-encoding mRNA including an RNA aptamer against the His-tag. When this system was tested using a sequence encoding His-tagged green fluorescent protein that included an RNA aptamer against the His-tag, the collection of mRNA encoding the protein was dependent on the RNA aptamer. Using this display method and a synthetic library of surrogate single-chain variable fragments consisting of VpreB and Ig heavy-chain variable domains, it was possible to isolate clones that could specifically recognize a particular target (intelectin-1 or tumor necrosis factor-α). These clones were obtained as soluble proteins produced by E. coli, and the purified peptide clones recognizing intelectin-1 could be used as detectors for sandwich enzyme-linked immunosorbent assays. The Hishot display will be a useful method to add to the repertoire of display technologies. |
| Volume | 8(12) |
| Pages | e83108 |
| Published | 2013-1-1 |
| DOI | 10.1371/journal.pone.0083108 |
| PII | PONE-D-13-33067 |
| PMID | 24386149 |
| PMC | PMC3873924 |
| MeSH | Amino Acid Sequence Base Sequence Cell Surface Display Techniques / methods* Enzyme-Linked Immunosorbent Assay / methods* Escherichia coli / genetics* Escherichia coli / metabolism Genetic Vectors Green Fluorescent Proteins / analysis Molecular Sequence Data Peptide Library |
| IF | 2.74 |
| Times Cited | 1 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | RK13(RCB0183) |