| RRC ID |
44832
|
| Author |
Tsuji S, Yamashita M, Kageyama T, Ohtsu T, Suzuki K, Kato S, Akitomi J, Furuichi M, Waga I.
|
| Title |
Hishot display--a new combinatorial display for obtaining target-recognizing peptides.
|
| Journal |
PLoS One
|
| Abstract |
Display technologies are procedures used for isolating target-recognizing peptides without using immunized animals. In this study, we describe a new display method, named Hishot display, that uses Escherichia coli and an expression plasmid to isolate target-recognizing peptides. This display method is based on the formation, in bacteria, of complexes between a polyhistidine (His)-tagged peptide including random sequences and the peptide-encoding mRNA including an RNA aptamer against the His-tag. When this system was tested using a sequence encoding His-tagged green fluorescent protein that included an RNA aptamer against the His-tag, the collection of mRNA encoding the protein was dependent on the RNA aptamer. Using this display method and a synthetic library of surrogate single-chain variable fragments consisting of VpreB and Ig heavy-chain variable domains, it was possible to isolate clones that could specifically recognize a particular target (intelectin-1 or tumor necrosis factor-α). These clones were obtained as soluble proteins produced by E. coli, and the purified peptide clones recognizing intelectin-1 could be used as detectors for sandwich enzyme-linked immunosorbent assays. The Hishot display will be a useful method to add to the repertoire of display technologies.
|
| Volume |
8(12)
|
| Pages |
e83108
|
| Published |
2013-1-1
|
| DOI |
10.1371/journal.pone.0083108
|
| PII |
PONE-D-13-33067
|
| PMID |
24386149
|
| PMC |
PMC3873924
|
| MeSH |
Amino Acid Sequence
Base Sequence
Cell Surface Display Techniques / methods*
Enzyme-Linked Immunosorbent Assay / methods*
Escherichia coli / genetics*
Escherichia coli / metabolism
Genetic Vectors
Green Fluorescent Proteins / analysis
Molecular Sequence Data
Peptide Library
|
| IF |
2.74
|
| Times Cited |
1
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
RK13(RCB0183) |
