RRC ID |
44836
|
Author |
Uto S, Nishizawa S, Takasawa Y, Asawa Y, Fujihara Y, Takato T, Hoshi K.
|
Title |
Bone and cartilage repair by transplantation of induced pluripotent stem cells in murine joint defect model.
|
Journal |
Biomed Res
|
Abstract |
The establishment of cartilage regenerative medicine has been an important issue in the clinical field, because cartilage has the poor ability of self-repair. Currently, tissue engineering using autologous chondrocytes has risen, but we should investigate more appropriate cell sources that can be obtained without any quantitative limitation. In this study, we focused on induced pluripotent stem (iPS) cells, in which the ethical hurdle does not seem higher than that of embryonic stem cells. Mouse iPS cells were transplanted into the mouse joint defect model of the knee. Strains of the transplants and hosts were arranged to be either closest (homology 75% in genetic background) or identical (100%). For transplantation, we embedded the iPS cells within the collagen hydrogel in order to obtain the effective administration of the cells into defects, which induced the differentiation of the iPS cells. At 8 weeks of transplantation, although the iPS cells with a 75% homology to the host in the genetic background tended to form teratoma, those of 100% showed a joint regeneration. GFP immunohistochemistry proved that the transplanted iPS cells were responsible for the bone and cartilage repair. Taking these results together, the iPS cells are regarded as a promising cell source for the cartilage tissue engineering.
|
Volume |
34(6)
|
Pages |
281-8
|
Published |
2013-1-1
|
DOI |
10.2220/biomedres.34.281
|
PII |
DN/JST.JSTAGE/biomedres/34.281
|
PMID |
24389404
|
MeSH |
Animals
Biomarkers / metabolism
Bone Regeneration / physiology*
Cartilage / pathology*
Cell Differentiation
Chondrocytes / cytology
Chondrocytes / metabolism
Collagen / chemistry
Gene Expression
Genes, Reporter
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
Histocompatibility Testing
Hydrogels
Induced Pluripotent Stem Cells / cytology
Induced Pluripotent Stem Cells / physiology
Induced Pluripotent Stem Cells / transplantation*
Knee Injuries / metabolism
Knee Injuries / pathology
Knee Injuries / therapy*
Knee Joint / pathology*
Mice
Mice, Inbred DBA
Mice, Transgenic
Teratoma / pathology
Wound Healing / physiology*
|
IF |
1.429
|
Times Cited |
21
|
WOS Category
|
MEDICINE, RESEARCH & EXPERIMENTAL
|
Resource |
Human and Animal Cells |
iPS-MEF-Ng-20D-17(APS0001) |