RRC ID 44844
Author Tan H, Furuta S, Nagata T, Ohnuki K, Akasaka T, Shirouchi B, Sato M, Kondo R, Shimizu K.
Title Inhibitory effects of the leaves of loquat (Eriobotrya japonica) on bone mineral density loss in ovariectomized mice and osteoclast differentiation.
Journal J Agric Food Chem
Abstract The loquat, Eriobotrya japonica Lindl. (Rosaceae), is a small tree native to Japan and China that is widely cultivated for its succulent fruit. Its leaves are used as an ingredient of a tasty tea called "Biwa cha" in Japanese. The anti-osteoporosis effects of the leaves of loquat in vitro and in vivo have been investigated. After 15 days of feeding normal diet or diet supplemented with 5% loquat leaves, the body weight, viscera weights, and bone mineral density (BMD) of both groups of eight ovariectomized (OVX) mice were compared. The result showed that the loss of BMD in loquat-fed mice was significantly prevented in three parts of the body, especially in the trabecular bone of the head (P < 0.05), abdomen (P < 0.01), and lumbar (P < 0.05) compared to the control group. No hypertrophy in the uterus by the loquat leaves diet was observed. The effect of the extract (447.25 g) prepared from the dried leaves of loquat (2.36 kg) was further studied on RANKL-induced osteoclast differentiation and cell viability. The extract suppressed the differentiation of osteoclasts under 50, 125, 250, and 500 μg/mL. Through bioactivity-guided fractionation, ursolic acid (1) was isolated and inhibited osteoclast differentiation under 4 and 10 μg/mL. It was concluded that loquat leaves possess the potential to suppress ovariectomy-induced bone mineral density deterioration.
Volume 62(4)
Pages 836-41
Published 2014-1-29
DOI 10.1021/jf402735u
PMID 24404864
MeSH Animals Bone Density / drug effects* Cell Differentiation / drug effects Eriobotrya / chemistry* Female Humans Japan Mice Mice, Inbred C57BL Osteoclasts / cytology Osteoclasts / drug effects* Osteoporosis, Postmenopausal / prevention & control* Ovariectomy Plant Extracts / pharmacology* Plant Leaves / chemistry*
IF 4.192
Times Cited 20
Human and Animal Cells RAW 264(RCB0535)