| RRC ID |
44852
|
| Author |
Yusuke Umezawa, Okihisa Kobayashi, Syoji Kanai, Masaru Hakoda
|
| Title |
Development of Particle Packed Bed Type Chromatography Using Dielectrophoresis
|
| Journal |
Key Engineering Materials
|
| Abstract |
In this study, we analyzed differences in cell species using dielectrophoresis (DEP) and propose a particle packed bed type chromatography device using DEP. Mouse-hybridoma 3-2H3 cells were used as the sample cells. The 3-2H3 cell suspension was fed pulse-wise to a carrier flow and the sample was measured by an absorption spectrometer. The analytical characteristic of chromatography using DEP was evaluated by the retention time difference.
We examined the effects of the diameter of packed particles on the retention time difference. The retention time difference in the case of the packed particles of 100 μm diameter was larger than that of 500 μm, which suggests that packed particles of smaller diameter are suitable for analysis using DEP chromatography.
|
| Volume |
534
|
| Pages |
88-92
|
| Published |
2013-1-11
|
| DOI |
10.4028/www.scientific.net/kem.534.88
|
| IF |
6.513
|
| Times Cited |
39
|
|
WOS Category
|
ACOUSTICS
CHEMISTRY, MULTIDISCIPLINARY
|
| Resource |
| Human and Animal Cells |
3-2H3(RCB0867) |
