RRC ID |
44895
|
Author |
Tsutsumi K, Saito N, Kawazoe Y, Ooi HK, Shiba T.
|
Title |
Morphogenetic study on the maturation of osteoblastic cell as induced by inorganic polyphosphate.
|
Journal |
PLoS One
|
Abstract |
Since inorganic polyphosphates [poly(P)] have an activity to induce bone differenciation in vitro and in vivo, we examined an effect of poly(P) on organelle by light microscopy and electron microscopy in Murine MC3T3-E1 osteoblastic cells. The MC3T3-E1 cells were ultrastructurally observed to possess morphological characteristics of osteoblasts. Cells cultured with poly(P) were strongly stained with an anti-collagen type I antibody but not in those cultured without poly(P). Ultrastructural analysis of cells cultured with poly(P) revealed a well-developed Golgi apparatus, swollen and elongated rough endoplasmic reticulum, large mitochondria and many coated pits. Since MC3T3-E1 cells can be transformed from a resting phase to an active blastic cell phase after supplementation with poly(P), it implies that poly(P) can be an effective material for bone regeneration.
|
Volume |
9(2)
|
Pages |
e86834
|
Published |
2014-1-1
|
DOI |
10.1371/journal.pone.0086834
|
PII |
PONE-D-13-34148
|
PMID |
24498284
|
PMC |
PMC3911941
|
MeSH |
Animals
Cell Differentiation / drug effects*
Cell Line
Collagen Type I / metabolism
Cytoplasm / drug effects
Cytoplasm / metabolism
Cytoplasm / ultrastructure
Mice
Microscopy, Electron, Transmission
Microscopy, Phase-Contrast
Osteoblasts / cytology
Osteoblasts / drug effects*
Osteoblasts / ultrastructure
Osteogenesis / drug effects*
Osteopontin / metabolism
Polyphosphates / pharmacology*
Time Factors
Vacuoles / drug effects
Vacuoles / ultrastructure
|
IF |
2.74
|
Times Cited |
25
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
Resource |
Human and Animal Cells |
MC3T3-E1(RCB1126) |