| RRC ID |
44908
|
| Author |
Kimura O, Ohta C, Koga N, Haraguchi K, Kato Y, Endo T.
|
| Title |
Carrier-mediated uptake of nobiletin, a citrus polymethoxyflavonoid, in human intestinal Caco-2 cells.
|
| Journal |
Food Chem
|
| Abstract |
The mechanism of intestinal absorption of nobiletin (NBL) was investigated using Caco-2 cells. The uptake of NBL from the apical membranes of Caco-2 cells was rapid and temperature-dependent and the presence of metabolic inhibitors, NaN3 and carbonylcyanide p-trifluoromethoxyphenylhydrazone, did not cause a decrease in NBL uptake. The relationship between the initial uptake of NBL and its concentration was saturable, suggesting the involvement of a carrier-mediated process. The Km and uptake clearance (Vmax/Km) values for NBL were 50.6 and 168.1μl/mg protein/min, respectively. This clearance value was about 9-fold greater than that of the non-saturable uptake clearance (Kd: 18.5μl/mg protein/min). The presence of structurally similar compounds, such as quercetin and luteolin, competitively inhibited NBL uptake. These results suggest that uptake of NBL from the apical membranes of Caco-2 cells is mainly mediated by an energy-independent facilitated diffusion process.
|
| Volume |
154
|
| Pages |
145-50
|
| Published |
2014-7-1
|
| DOI |
10.1016/j.foodchem.2013.12.069
|
| PII |
S0308-8146(13)01937-7
|
| PMID |
24518326
|
| MeSH |
Biological Transport
Caco-2 Cells
Citrus / metabolism*
Flavones / chemistry
Flavones / metabolism*
Flavonoids / metabolism*
Humans
Intestinal Absorption
Intestinal Mucosa / metabolism*
Intestines / chemistry
Kinetics
Plant Extracts / chemistry
Plant Extracts / metabolism*
|
| IF |
6.306
|
| Times Cited |
9
|
|
WOS Category
|
FOOD SCIENCE & TECHNOLOGY
NUTRITION & DIETETICS
CHEMISTRY, APPLIED
|
| Resource |
| Human and Animal Cells |
CACO-2(RCB0988) |
