論文 - 詳細
| RRC ID | 4491 |
|---|---|
| 著者 | Auesukaree C, Damnernsawad A, Kruatrachue M, Pokethitiyook P, Boonchird C, Kaneko Y, Harashima S. |
| タイトル | Genome-wide identification of genes involved in tolerance to various environmental stresses in Saccharomyces cerevisiae. |
| ジャーナル | J Appl Genet |
| Abstract |
During fermentation, yeast cells are exposed to a number of stresses -- such as high alcohol concentration, high osmotic pressure, and temperature fluctuation - so some overlap of mechanisms involved in the response to these stresses has been suggested. To identify the genes required for tolerance to alcohol (ethanol, methanol, and 1-propanol), heat, osmotic stress, and oxidative stress, we performed genome-wide screening by using 4828 yeast deletion mutants. Our screens identified 95, 54, 125, 178, 42, and 30 deletion mutants sensitive to ethanol, methanol, 1-propanol, heat, NaCl, and H2O2, respectively. These deleted genes were then classified based on their cellular functions, and cross-sensitivities between stresses were determined. A large number of genes involved in vacuolar H(+)-ATPase (V-ATPase) function, cytoskeleton biogenesis, and cell wall integrity, were required for tolerance to alcohol, suggesting their protective role against alcohol stress. Our results revealed a partial overlap between genes required for alcohol tolerance and those required for thermotolerance. Genes involved in cell wall integrity and the actin cytoskeleton are required for both alcohol tolerance and thermotolerance, whereas the RNA polymerase II mediator complex seems to be specific to heat tolerance. However, no significant overlap of genes required for osmotic stress and oxidative stress with those required for other stresses was observed. Interestingly, although mitochondrial function is likely involved in tolerance to several stresses, it was found to be less important for thermotolerance. The genes identified in this study should be helpful for future research into the molecular mechanisms of stress response. |
| 巻・号 | 50(3) |
| ページ | 301-10 |
| 公開日 | 2009-1-1 |
| DOI | 10.1007/BF03195688 |
| PII | 523 |
| PMID | 19638689 |
| MeSH | 1-Propanol / pharmacology DNA, Fungal / drug effects DNA, Fungal / genetics* Ethanol / pharmacology Gene Expression Regulation, Fungal / drug effects Gene Expression Regulation, Fungal / genetics* Genome, Fungal / drug effects Genome, Fungal / genetics* Genome-Wide Association Study Hydrogen Peroxide / pharmacology Methanol / pharmacology Osmotic Pressure / physiology Oxidative Stress / genetics Saccharomyces cerevisiae / drug effects Saccharomyces cerevisiae / genetics* Sequence Deletion / drug effects Sequence Deletion / genetics Stress, Physiological / genetics* |
| IF | 2.027 |
| 引用数 | 96 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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