論文 - 詳細
| RRC ID | 44923 |
|---|---|
| 著者 | Kim H, Tabata A, Tomoyasu T, Ueno T, Uchiyama S, Yuasa K, Tsuji A, Nagamune H. |
| タイトル | Estrogen stimuli promote osteoblastic differentiation via the subtilisin-like proprotein convertase PACE4 in MC3T3-E1 cells. |
| ジャーナル | J Bone Miner Metab |
| Abstract |
Estrogenic compounds include endogenous estrogens such as estradiol as well as soybean isoflavones, such as daidzein and its metabolite equol, which are known phytoestrogens that prevent osteoporosis in postmenopausal women. Indeed, mineralization of MC3T3-E1 cells, a murine osteoblastic cell line, was significantly decreased in medium containing fetal bovine serum treated with charcoal-dextran to deplete endogenous estrogens, but estradiol and these soybean isoflavones dose-dependently restored the differentiation of MC3T3-E1 cells; equol was tenfold more effective than daidzein. These differentiation-promoting effects were inhibited by the addition of fulvestrant, which is a selective downregulator of estrogen receptors. Analysis of the expression pattern of bone-related genes by reverse transcription PCR (RT-PCR)/quantitative real-time PCR (qRT-PCR), which focused on responsiveness to the estrogen stimuli, revealed that the transcription of PACE4, a subtilisin-like proprotein convertase, was tightly linked with the differentiation of MC3T3-E1 cells induced by estrogen stimuli. Moreover, treatment with RNAi of PACE4 in MC3T3-E1 cells resulted in a drastic decrease of mineralization in the presence of estrogen stimuli. These results strongly suggest that PACE4 participates in bone formation at least in osteoblast differentiation, and estrogen receptor-mediated stimuli induce osteoblast differentiation through the upregulation of PACE4 expression. |
| 巻・号 | 33(1) |
| ページ | 30-9 |
| 公開日 | 2015-1-1 |
| DOI | 10.1007/s00774-014-0567-9 |
| PMID | 24557631 |
| MeSH | 3T3 Cells Animals Bone and Bones / metabolism Cell Differentiation Charcoal / chemistry Chondrocytes / cytology Culture Media / chemistry Dextrans / chemistry Estradiol / analogs & derivatives Estradiol / chemistry Estrogens / metabolism* Female Fulvestrant Isoflavones / chemistry Mice Osteoblasts / cytology* Proprotein Convertases / metabolism* Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Soybeans Subtilisin / chemistry* Transcription, Genetic |
| IF | 2.297 |
| 引用数 | 11 |
| WOS 分野 | MEDICINE, RESEARCH & EXPERIMENTAL ENDOCRINOLOGY & METABOLISM |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |