論文 - 詳細
| RRC ID | 4493 |
|---|---|
| 著者 | Chien CY, Chou CK, Su JY. |
| タイトル | Ung1p-mediated uracil-base excision repair in mitochondria is responsible for the petite formation in thymidylate deficient yeast. |
| ジャーナル | FEBS Lett |
| Abstract |
The budding yeast CDC21 gene, which encodes thymidylate synthase, is crucial in the thymidylate biosynthetic pathway. Early studies revealed that high frequency of petites were formed in heat-sensitive cdc21 mutants grown at the permissive temperature. However, the molecular mechanism involved in such petite formation is largely unknown. Here we used a yeast cdc21-1 mutant to demonstrate that the mutant cells accumulated dUMP in the mitochondrial genome. When UNG1 (encoding uracil-DNA glycosylase) was deleted from cdc21-1, we found that the ung1Delta cdc21-1 double mutant reduced frequency of petite formation to the level found in wild-type cells. We propose that the initiation of Ung1p-mediated base excision repair in the uracil-laden mitochondrial genome in a cdc21-1 mutant is responsible for the mitochondrial petite mutations. |
| 巻・号 | 583(9) |
| ページ | 1499-504 |
| 公開日 | 2009-5-6 |
| DOI | 10.1016/j.febslet.2009.04.003 |
| PII | S0014-5793(09)00277-4 |
| PMID | 19362086 |
| MeSH | DNA Repair / physiology* DNA, Fungal / metabolism DNA, Mitochondrial / genetics DNA, Mitochondrial / metabolism* Flow Cytometry S Phase Saccharomyces cerevisiae / enzymology Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / metabolism* Thymidine Monophosphate / metabolism* Uracil / metabolism* Uracil-DNA Glycosidase / metabolism Uracil-DNA Glycosidase / physiology* |
| IF | 3.057 |
| 引用数 | 1 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY BIOPHYSICS CELL BIOLOGY |
| リソース情報 | |
| 酵母 | BY20612 |