| RRC ID |
44942
|
| 著者 |
Ono H, Motoi N, Nagano H, Miyauchi E, Ushijima M, Matsuura M, Okumura S, Nishio M, Hirose T, Inase N, Ishikawa Y.
|
| タイトル |
Long noncoding RNA HOTAIR is relevant to cellular proliferation, invasiveness, and clinical relapse in small-cell lung cancer.
|
| ジャーナル |
Cancer Med
|
| Abstract |
Small-cell lung cancer (SCLC) is a subtype of lung cancer with poor prognosis. To identify accurate predictive biomarkers and effective therapeutic modalities, we focus on a long noncoding RNA, Hox transcript antisense intergenic RNA (HOTAIR), and investigated its expression, cellular functions, and clinical relevance in SCLC. In this study, HOTAIR expression was assessed in 35 surgical SCLC samples and 10 SCLC cell lines. The efficacy of knockdown of HOTAIR by siRNA transfection was evaluated in SBC-3 cells in vitro, and the gene expression was analyzed using microarray. HOTAIR was expressed highly in pure, rather than combined, SCLC (P = 0.012), that the subgroup with high expression had significantly more pure SCLC (P = 0.04), more lymphatic invasion (P = 0.03) and more relapse (P = 0.04) than the low-expression subgroup. The knockdown of HOTAIR in SBC-3 cells led to decreased proliferation activity and decreased invasiveness in vitro. Gene expression analysis indicated that depletion of HOTAIR resulted in upregulation of cell adhesion-related genes such as ASTN1, PCDHA1, and mucin production-related genes such as MUC5AC, and downregulation of genes involved in neuronal growth and signal transduction including NTM and PTK2B. Our results suggest that HOTAIR has an oncogenic role in SCLC and could be a prognostic biomarker and therapeutic target.
|
| 巻・号 |
3(3)
|
| ページ |
632-42
|
| 公開日 |
2014-6-1
|
| DOI |
10.1002/cam4.220
|
| PMID |
24591352
|
| PMC |
PMC4101754
|
| MeSH |
Aged
Cell Line, Tumor
Cell Proliferation / genetics*
Female
Humans
Male
Middle Aged
Neoplasm Invasiveness / genetics*
RNA, Long Noncoding / biosynthesis
RNA, Long Noncoding / genetics*
Recurrence
Small Cell Lung Carcinoma / genetics*
Small Cell Lung Carcinoma / pathology
|
| IF |
3.491
|
| 引用数 |
105
|
|
WOS 分野
|
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
