論文 - 詳細
| RRC ID | 44946 |
|---|---|
| 著者 | Nishikawa R, Anada T, Ishiko-Uzuka R, Suzuki O. |
| タイトル | Osteoblastic differentiation of stromal ST-2 cells from octacalcium phosphate exposure via p38 signaling pathway. |
| ジャーナル | Dent Mater J |
| Abstract |
The present study investigated the role of mitogen-activated protein kinase (MAPK) signaling in osteoblastic differentiation of stromal ST-2 cells induced by synthetic octacalcium phosphate (OCP) incubation. Since our previous studies revealed that OCP consumes calcium ions in media during conversion to hydroxyapatite, the effect of the ions on ST-2 cell differentiation with or without OCP crystals was analyzed. The effect of presence or absence of MAPK inhibitors was also analyzed. OCP increased alkaline phosphatase (ALP) activity and the mRNA expression of differentiation markers via the p38 signaling pathway. The PD98059 MAPK inhibitor increased ALP activity and differentiation marker genes in cells cultured in OCP-coated wells. Reduction of calcium ions in the medium by EGTA increased the ALP activity without OCP in the presence of phosphate ion concentrations up to 7.5 mM. OCP may enhance osteoblastic differentiation through the p38 signaling pathway via the reduction of calcium ions induced by its physicochemical property. |
| 巻・号 | 33(2) |
| ページ | 242-51 |
| 公開日 | 2014-1-1 |
| DOI | 10.4012/dmj.2013-226 |
| PII | DN/JST.JSTAGE/dmj/2013-226 |
| PMID | 24598239 |
| MeSH | Alkaline Phosphatase / metabolism Androstadienes / pharmacology Animals Calcium Phosphates / chemistry Calcium Phosphates / pharmacology* Cell Differentiation / drug effects* Cell Line Cell Proliferation / drug effects Flavonoids / pharmacology Imidazoles / pharmacology Mice Molecular Structure Osteoblasts / cytology* Pyridines / pharmacology Signal Transduction* Stromal Cells / cytology* Stromal Cells / enzymology Wortmannin p38 Mitogen-Activated Protein Kinases / antagonists & inhibitors p38 Mitogen-Activated Protein Kinases / metabolism* |
| IF | 1.359 |
| 引用数 | 10 |
| WOS 分野 | DENTISTRY, ORAL SURGERY & MEDICINE MATERIALS SCIENCE, BIOMATERIALS |
| リソース情報 | |
| ヒト・動物細胞 | ST2(RCB0224) |