Reference - Detail
| RRC ID | 44979 |
|---|---|
| Author | Ozaki Y, Takagi Y, Saito Y, Mori H, Hara M. |
| Title | Purification of porcine hair keratin subunits and their immobilization for use as cell culture substrates. |
| Journal | Biosci Biotechnol Biochem |
| Abstract |
We purified both the type I subunit and type II subunit of porcine hair keratin and compared their ability to form a uniform film of reconstituted keratin on a culture plate, and their effect on a model of neural cells. We observed the surface of the keratin-immobilized plate using a scanning electron microscope (SEM) and measured water contact angles to characterize the surface. We cultured PC12 cells on plates on which crude keratin, the type I subunit, or the type II subunit were immobilized. The water contact angles were slightly different from each other. The cells proliferated well on all three keratin-immobilized plates. The type II subunit showed a tendency to inhibit the differentiation of PC12 cells significantly as an extension of the cell shapes and neurite outgrowth in comparison with the crude extract and the type I subunit. The type I subunit and the type II subunit showed slight differences in cell differentiation, but not in cell proliferation. |
| Volume | 77(9) |
| Pages | 1894-900 |
| Published | 2013-1-1 |
| DOI | 10.1271/bbb.130339 |
| PII | DN/JST.JSTAGE/bbb/130339 |
| PMID | 24018666 |
| MeSH | Amino Acid Sequence Animals Cell Culture Techniques / methods* Cell Differentiation / drug effects Cell Proliferation / drug effects Hair / chemistry* Immobilized Proteins / chemistry* Immobilized Proteins / isolation & purification Immobilized Proteins / metabolism* Immobilized Proteins / pharmacology Keratins / chemistry* Keratins / isolation & purification Keratins / metabolism* Keratins / pharmacology Keratins, Type I / chemistry Keratins, Type I / isolation & purification Keratins, Type I / metabolism Keratins, Type I / pharmacology Keratins, Type II / chemistry Keratins, Type II / isolation & purification Keratins, Type II / metabolism Keratins, Type II / pharmacology Molecular Sequence Data PC12 Cells Protein Stability Rats Surface Properties Swine* |
| IF | 2.74 |
| Times Cited | 13 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | PC-12(RCB0009) |