| RRC ID |
44979
|
| Author |
Ozaki Y, Takagi Y, Saito Y, Mori H, Hara M.
|
| Title |
Purification of porcine hair keratin subunits and their immobilization for use as cell culture substrates.
|
| Journal |
Biosci Biotechnol Biochem
|
| Abstract |
We purified both the type I subunit and type II subunit of porcine hair keratin and compared their ability to form a uniform film of reconstituted keratin on a culture plate, and their effect on a model of neural cells. We observed the surface of the keratin-immobilized plate using a scanning electron microscope (SEM) and measured water contact angles to characterize the surface. We cultured PC12 cells on plates on which crude keratin, the type I subunit, or the type II subunit were immobilized. The water contact angles were slightly different from each other. The cells proliferated well on all three keratin-immobilized plates. The type II subunit showed a tendency to inhibit the differentiation of PC12 cells significantly as an extension of the cell shapes and neurite outgrowth in comparison with the crude extract and the type I subunit. The type I subunit and the type II subunit showed slight differences in cell differentiation, but not in cell proliferation.
|
| Volume |
77(9)
|
| Pages |
1894-900
|
| Published |
2013-1-1
|
| DOI |
10.1271/bbb.130339
|
| PII |
DN/JST.JSTAGE/bbb/130339
|
| PMID |
24018666
|
| MeSH |
Amino Acid Sequence
Animals
Cell Culture Techniques / methods*
Cell Differentiation / drug effects
Cell Proliferation / drug effects
Hair / chemistry*
Immobilized Proteins / chemistry*
Immobilized Proteins / isolation & purification
Immobilized Proteins / metabolism*
Immobilized Proteins / pharmacology
Keratins / chemistry*
Keratins / isolation & purification
Keratins / metabolism*
Keratins / pharmacology
Keratins, Type I / chemistry
Keratins, Type I / isolation & purification
Keratins, Type I / metabolism
Keratins, Type I / pharmacology
Keratins, Type II / chemistry
Keratins, Type II / isolation & purification
Keratins, Type II / metabolism
Keratins, Type II / pharmacology
Molecular Sequence Data
PC12 Cells
Protein Stability
Rats
Surface Properties
Swine*
|
| IF |
2.74
|
| Times Cited |
13
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
PC-12(RCB0009) |
