論文 - 詳細
| RRC ID | 44987 |
|---|---|
| 著者 | Yamada H, Oshiro E, Kikuchi S, Hakozaki M, Takahashi H, Kimura K. |
| タイトル | Hydroxyeicosapentaenoic acids from the Pacific krill show high ligand activities for PPARs. |
| ジャーナル | J Lipid Res |
| Abstract |
PPARs regulate the expression of genes for energy metabolism in a ligand-dependent manner. PPARs can influence fatty acid oxidation, the level of circulating triglycerides, glucose uptake and insulin sensitivity. Here, we demonstrate that 5-hydroxyeicosapentaenoic acid (HEPE), 8-HEPE, 9-HEPE, 12-HEPE and 18-HEPE (hydroxylation products of EPA) obtained from methanol extracts of Pacific krill (Euphausia pacifica) can act as PPAR ligands. Two of these products, 8-HEPE and 9-HEPE, enhanced the transcription levels of GAL4-PPARs to a significantly greater extent than 5-HEPE, 12-HEPE, 18-HEPE, EPA, and EPA ethyl-ester. 8-HEPE also activated significantly higher transcription of GAL4-PPARα, GAL4-PPARγ, and GAL4-PPARδ than EPA at concentrations greater than 4, 64, and 64 μM, respectively. We also demonstrated that 8-HEPE increased the expression levels of genes regulated by PPARs in FaO, 3T3-F442A, and C2C12 cells. Furthermore, 8-HEPE enhanced adipogenesis and glucose uptake. By contrast, at the same concentrations, EPA showed weak or little effect, indicating that 8-HEPE was the more potent inducer of physiological effects. |
| 巻・号 | 55(5) |
| ページ | 895-904 |
| 公開日 | 2014-5-1 |
| DOI | 10.1194/jlr.M047514 |
| PII | S0022-2275(20)37508-8 |
| PMID | 24668940 |
| PMC | PMC3995467 |
| MeSH | Adipogenesis / drug effects Angiopoietin-Like Protein 4 Angiopoietins / genetics Animals Biological Transport / drug effects Cell Line Eicosapentaenoic Acid / metabolism* Eicosapentaenoic Acid / pharmacology Euphausiacea / chemistry* Fatty Acids / metabolism Glucose / metabolism Humans Ligands Mice Mitochondria / drug effects Mitochondria / metabolism Myoblasts / drug effects Myoblasts / metabolism Oxidation-Reduction Peroxisome Proliferator-Activated Receptors / genetics Peroxisome Proliferator-Activated Receptors / metabolism* Peroxisomes / drug effects Peroxisomes / metabolism Transcriptional Activation / drug effects |
| IF | 4.483 |
| 引用数 | 22 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | C2C12(RCB0987) NIH3T3 |