論文 - 詳細
| RRC ID | 44998 |
|---|---|
| 著者 | Nomoto T, Fukushima S, Kumagai M, Machitani K, Arnida, Matsumoto Y, Oba M, Miyata K, Osada K, Nishiyama N, Kataoka K. |
| タイトル | Three-layered polyplex micelle as a multifunctional nanocarrier platform for light-induced systemic gene transfer. |
| ジャーナル | Nat Commun |
| Abstract |
Nanocarriers responding to light have great potential for pinpoint therapy, and recent studies have revealed promising in vivo activity. However, light-selective gene transfer still remains challenging in the systemic application. Here we report systemic light-responsive nanocarriers for gene delivery developed through the sequential self-assembly of ABC-type triblock copolymer/DNA/dendrimeric photosensitizer, forming polyplex micelles with three-layered functional nanocompartments. The DNA-packaged core is covered by the photosensitizer-incorporated intermediate layer, which is encompassed by an outer shielding shell. This three-layered structure permits multistep photosensitizer and DNA delivery into a solid tumour by a systemic route: the shielding layer minimizes unfavourable interactions with blood components, and the photosensitizer is delivered to endo-/lysosomal membranes to facilitate light-selective cytoplasmic translocation of the micelles, accomplishing DNA delivery into the nucleus to exert gene expression. The polyplex micelles display >100-fold photoenhanced gene expression in cultured cells and exhibit light-induced in vivo gene transfer in solid tumours following systemic administration. |
| 巻・号 | 5 |
| ページ | 3545 |
| 公開日 | 2014-4-2 |
| DOI | 10.1038/ncomms4545 |
| PII | ncomms4545 |
| PMID | 24694458 |
| MeSH | Animals Cell Line, Tumor DNA / chemistry DNA / genetics* Female Genetic Therapy / instrumentation* Genetic Therapy / methods Humans Light Mice Mice, Inbred BALB C Mice, SCID Micelles Nanoparticles / chemistry* Photosensitizing Agents / chemistry Polymers / chemistry* Transfection / instrumentation* Transfection / methods |
| IF | 12.121 |
| 引用数 | 122 |
| WOS 分野 | PHARMACOLOGY & PHARMACY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa |