論文 - 詳細
| RRC ID | 45014 |
|---|---|
| 著者 | Hanaki K, Ike F, Kajita A, Yasuno W, Yanagiba M, Goto M, Sakai K, Ami Y, Kyuwa S. |
| タイトル | Detection of murine norovirus by reverse transcription loop-mediated isothermal amplification. |
| ジャーナル | J Virol Methods |
| Abstract |
Murine norovirus (MNV) has considerable genetical and biological diversity and is recognized worldwide as the most common contaminant in laboratory mouse colonies. This study developed a reverse transcription loop-mediated isothermal amplification (RT-LAMP) method with the potential to detect a broad range of MNV. RT-LAMP, using a set of five primers containing mixed bases, obtained results under isothermal conditions at 62°C for 90min. Sensitivity of RT-LAMP was 50-fold less than that of two-step TaqMan real-time reverse transcription-polymerase chain reaction (TaqMan RT-PCR). Diagnostic performance of RT-LAMP on RNA extracted from mouse fecal specimens was compared with TaqMan RT-PCR and nested RT-PCR. MNV was detected in 54 of 120 mouse fecal specimens by RT-LAMP, and RT-LAMP had an estimated sensitivity and specificity of 96.4% and 100% compared with TaqMan RT-PCR, and 94.7% and 100% compared with nested RT-PCR. RT-LAMP, which does not require expensive instruments, might be useful for the screening of mice actively or persistently infected with MNV. |
| 巻・号 | 204 |
| ページ | 17-24 |
| 公開日 | 2014-8-1 |
| DOI | 10.1016/j.jviromet.2014.03.025 |
| PII | S0166-0934(14)00131-1 |
| PMID | 24717164 |
| PMC | PMC7172776 |
| MeSH | Animals Caliciviridae Infections / veterinary* Caliciviridae Infections / virology DNA Primers / genetics Feces / virology Mice Norovirus / genetics Norovirus / isolation & purification* Nucleic Acid Amplification Techniques / methods* Reverse Transcription* Rodent Diseases / diagnosis* Rodent Diseases / virology* Sensitivity and Specificity Temperature Time Factors |
| IF | 1.786 |
| 引用数 | 5 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMICAL RESEARCH METHODS VIROLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |