| RRC ID |
45015
|
| 著者 |
Ninomiya K, Fukuda A, Ogino C, Shimizu N.
|
| タイトル |
Targeted sonocatalytic cancer cell injury using avidin-conjugated titanium dioxide nanoparticles.
|
| ジャーナル |
Ultrason Sonochem
|
| Abstract |
In this study, we applied sonodynamic therapy to cancer cells based on the delivery of titanium dioxide (TiO2) nanoparticles (NPs) modified with avidin protein, which preferentially discriminated cancerous cells from healthy cells. Subsequently, hydroxyl radicals were generated from the TiO2 NPs after activation by external ultrasound irradiation (TiO2/US treatment). Although 30% of the normal breast cells (human mammary epithelial cells) exhibited the uptake of avidin-modified TiO2 NPs, over 80% of the breast cancer cells (MCF-7) exhibited the uptake of avidin-TiO2 NPs. Next the effect of the TiO2/US treatment on MCF-7 cell growth was examined for up to 96 h after 1-MHz ultrasound was applied (0.1 W/cm(2), 30 s) to cells that incorporated the TiO2 NPs. No apparent cell injury was observed until 24h after the treatment, but the viable cell concentration declined to 68% compared with the control at 96 h.
|
| 巻・号 |
21(5)
|
| ページ |
1624-8
|
| 公開日 |
2014-9-1
|
| DOI |
10.1016/j.ultsonch.2014.03.010
|
| PII |
S1350-4177(14)00099-6
|
| PMID |
24717690
|
| MeSH |
Avidin / chemistry*
Avidin / therapeutic use*
Breast Neoplasms / drug therapy
Breast Neoplasms / pathology
Cell Line, Tumor
Female
Flow Cytometry
Humans
MCF-7 Cells
Nanoparticles / chemistry*
Nanoparticles / therapeutic use*
Neoplasms / therapy*
Titanium / chemistry*
Titanium / therapeutic use*
Ultrasonic Therapy / methods*
|
| IF |
6.513
|
| 引用数 |
23
|
|
WOS 分野
|
ACOUSTICS
CHEMISTRY, MULTIDISCIPLINARY
|
| リソース情報 |
| ヒト・動物細胞 |
MCF7(RCB1904) |
