| RRC ID |
45040
|
| Author |
Kobayashi M, Nagashio R, Ryuge S, Murakami Y, Yanagita K, Nakashima H, Matsumoto T, Jiang SX, Saegusa M, Satoh Y, Masuda N, Sato Y.
|
| Title |
Acquisition of useful sero-diagnostic autoantibodies using the same patients'sera and tumor tissues.
|
| Journal |
Biomed Res
|
| Abstract |
Cancer tissues are comprised of various components including tumor cells and the surrounding tumor stroma, which consists of the extracellular matrix and inflammatory cells. Since the tumor stroma plays critical roles in tumor development, investigation of the tumor stroma in addition to tumor cells is important to identify useful tumor-associated markers. To discover novel and useful sero-diagnostic markers, a comparative study of tumor-associated autoantibodies (AAbs) in sera from lung adenocarcinoma (AC) patients was investigated by two-dimensional immunoblotting with AC cell lines or each autologous AC tissues. Autoantigens identified from tissue and cell line samples comprised 58 (45 antigens) and 53 spots (41 antigens), respectively. Thirty-six proteins including Transforming growth factor-beta-induced protein ig-h3 (BIGH3) and Hyaluronan and proteoglycan link protein 1 (HAPLN1) were detected only from tissues, 32 proteins only from cell lines, and 9 proteins from both. BIGH3 and HAPLN1 expressions were confirmed in the tumor stroma, but not in AC cell lines by immunostaining and immunoblotting. These data suggest that autologous tumor tissue and serum are important to coincidently detect AAbs derived from the tumor stroma in addition to tumor cells.
|
| Volume |
35(2)
|
| Pages |
133-43
|
| Published |
2014-1-1
|
| DOI |
10.2220/biomedres.35.133
|
| PII |
DN/JST.JSTAGE/biomedres/35.133
|
| PMID |
24759181
|
| MeSH |
Adenocarcinoma / blood
Adenocarcinoma / immunology
Adenocarcinoma / metabolism
Adenocarcinoma of Lung
Aged
Annexin A2 / immunology
Annexin A2 / metabolism
Antigens, Neoplasm / immunology
Antigens, Neoplasm / metabolism
Autoantibodies / blood
Autoantibodies / immunology*
Autoantigens / immunology
Autoantigens / metabolism
Biomarkers, Tumor / blood
Biomarkers, Tumor / immunology*
Biomarkers, Tumor / metabolism
Cell Line, Tumor
Electrophoresis, Gel, Two-Dimensional
Extracellular Matrix Proteins / immunology
Extracellular Matrix Proteins / metabolism
Female
Humans
Immunohistochemistry
Lung Neoplasms / blood
Lung Neoplasms / immunology
Lung Neoplasms / metabolism
Male
Microfilament Proteins / immunology
Microfilament Proteins / metabolism
Middle Aged
Neoplasms / blood
Neoplasms / diagnosis*
Neoplasms / immunology*
Neoplasms / metabolism
Nuclear Proteins / immunology
Nuclear Proteins / metabolism
Proteoglycans / immunology
Proteoglycans / metabolism
Transforming Growth Factor beta / immunology
Transforming Growth Factor beta / metabolism
|
| IF |
1.429
|
| Times Cited |
3
|
|
WOS Category
|
MEDICINE, RESEARCH & EXPERIMENTAL
|
| Resource |
| Human and Animal Cells |
LC-2/ad(RCB0440)
RERF-LC-AI(RCB0444) |
