論文 - 詳細
| RRC ID | 45048 |
|---|---|
| 著者 | Ohnuki Y, Kurosawa H. |
| タイトル | Effects of hanging drop culture conditions on embryoid body formation and neuronal cell differentiation using mouse embryonic stem cells: optimization of culture conditions for the formation of well-controlled embryoid bodies. |
| ジャーナル | J Biosci Bioeng |
| Abstract |
Hanging drop (HD) cultures were carried out with a drop volume of either 20 or 30 μl. An incubation period of 3 days was determined to be appropriate for the formation of well-controlled embryoid bodies (EBs), and the initial cell number was identified as the most critical factor in the growth and neuronal cell differentiation of EBs. |
| 巻・号 | 115(5) |
| ページ | 571-4 |
| 公開日 | 2013-5-1 |
| DOI | 10.1016/j.jbiosc.2012.11.016 |
| PII | S1389-1723(12)00467-7 |
| PMID | 23276518 |
| MeSH | Animals Cell Culture Techniques* Cell Line Embryoid Bodies / cytology* Embryonic Stem Cells / cytology Mice Neurogenesis* Neurons / cytology* |
| IF | 15.302 |
| 引用数 | 36 |
| WOS 分野 | ONCOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | STO(RCB0536) |