論文 - 詳細
| RRC ID | 45054 |
|---|---|
| 著者 | Haniu H, Saito N, Matsuda Y, Tsukahara T, Usui Y, Maruyama K, Takanashi S, Aoki K, Kobayashi S, Nomura H, Tanaka M, Okamoto M, Kato H. |
| タイトル | Biological responses according to the shape and size of carbon nanotubes in BEAS-2B and MESO-1 cells. |
| ジャーナル | Int J Nanomedicine |
| Abstract |
This study aimed to investigate the influence of the shape and size of multi-walled carbon nanotubes (MWCNTs) and cup-stacked carbon nanotubes (CSCNTs) on biological responses in vitro. Three types of MWCNTs - VGCF(®)-X, VGCF(®)-S, and VGCF(®) (vapor grown carbon fibers; with diameters of 15, 80, and 150 nm, respectively) - and three CSCNTs of different lengths (CS-L, 20-80 μm; CS-S, 0.5-20 μm; and CS-M, of intermediate length) were tested. Human bronchial epithelial (BEAS-2B) and malignant pleural mesothelioma cells were exposed to the CNTs (1-50 μg/mL), and cell viability, permeability, uptake, total reactive oxygen species/superoxide production, and intracellular acidity were measured. CSCNTs were less toxic than MWCNTs in both cell types over a 24-hour exposure period. The cytotoxicity of endocytosed MWCNTs varied according to cell type/size, while that of CSCNTs depended on tube length irrespective of cell type. CNT diameter and length influenced cell aggregation and injury extent. Intracellular acidity increased independently of lysosomal activity along with the number of vacuoles in BEAS-2B cells exposed for 24 hours to either CNT (concentration, 10 μg/mL). However, total reactive oxygen species/superoxide generation did not contribute to cytotoxicity. The results demonstrate that CSCNTs could be suitable for biological applications and that CNT shape and size can have differential effects depending on cell type, which can be exploited in the development of highly specialized, biocompatible CNTs. |
| 巻・号 | 9 |
| ページ | 1979-90 |
| 公開日 | 2014-1-1 |
| DOI | 10.2147/IJN.S58661 |
| PII | ijn-9-1979 |
| PMID | 24790438 |
| PMC | PMC4000181 |
| MeSH | Biocompatible Materials / chemistry Biocompatible Materials / toxicity* Cell Line Cell Survival / drug effects* Dose-Response Relationship, Drug Humans Materials Testing Nanotubes, Carbon / chemistry Nanotubes, Carbon / toxicity* Nanotubes, Carbon / ultrastructure* Oxidative Stress / drug effects* Particle Size Structure-Activity Relationship |
| IF | 5.115 |
| 引用数 | 20 |
| WOS 分野 | PHARMACOLOGY & PHARMACY NANOSCIENCE & NANOTECHNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Podcasts |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | ACC-MESO-1(RCB2292) |