| RRC ID |
45061
|
| Author |
Satoh T, Sugiura S, Sumaru K, Ozaki S, Gomi S, Kurakazu T, Oshima Y, Kanamori T.
|
| Title |
Inverting microwell array chip for the cultivation of human induced pluripotent stem cells with controlled aggregate size and geometrical arrangement.
|
| Journal |
Biomicrofluidics
|
| Abstract |
We present a novel cell culture chip, namely, "inverting microwell array chip," for cultivation of human induced pluripotent stem cells. The chip comprises a lower hydrogel microwell array and an upper polystyrene culture surface. We demonstrate the formation of uniform cellular aggregates in the microwell array, and after inversion, a culture with controlled aggregate size and geometrical arrangement on the polystyrene surface. Here, we report effects of cell concentrations on a cultivation sequence in the chip.
|
| Volume |
8(2)
|
| Pages |
024112
|
| Published |
2014-3-1
|
| DOI |
10.1063/1.4871036
|
| PII |
013402BMF
|
| PMID |
24803961
|
| PMC |
PMC4000399
|
| IF |
2.504
|
| Times Cited |
2
|
|
WOS Category
|
PHYSICS, FLUIDS & PLASMAS
BIOCHEMICAL RESEARCH METHODS
BIOPHYSICS
NANOSCIENCE & NANOTECHNOLOGY
|
| Resource |
| Human and Animal Cells |
201B7(HPS0063) |
