RRC ID 45062
Author Samet I, Han J, Jlaiel L, Sayadi S, Isoda H.
Title Olive (Olea europaea) leaf extract induces apoptosis and monocyte/macrophage differentiation in human chronic myelogenous leukemia K562 cells: insight into the underlying mechanism.
Journal Oxid Med Cell Longev
Abstract Differentiation therapy is an attractive approach aiming at reversing malignancy and reactivating endogenous differentiation programs in cancer cells. Olive leaf extract, known for its antioxidant activity, has been demonstrated to induce apoptosis in several cancer cells. However, its differentiation inducing properties and the mechanisms involved are still poorly understood. In this study, we investigated the effect of Chemlali Olive Leaf Extract (COLE) for its potential differentiation inducing effect on multipotent leukemia K562 cells. Results showed that COLE inhibits K562 cells proliferation and arrests the cell cycle at G0/G1, and then at G2/M phase over treatment time. Further analysis revealed that COLE induces apoptosis and differentiation of K562 cells toward the monocyte lineage. Microarray analysis was conducted to investigate the underlying mechanism of COLE differentiation inducing effect. The differentially expressed genes such as IFI16, EGR1, NFYA, FOXP1, CXCL2, CXCL3, and CXCL8 confirmed the commitment of K562 cells to the monocyte/macrophage lineage. Thus our results provide evidence that, in addition to apoptosis, induction of differentiation is one of the possible therapeutic effects of olive leaf in cancer cells.
Volume 2014
Pages 927619
Published 2014
DOI 10.1155/2014/927619
PMID 24803988
PMC PMC3997986
MeSH Antineoplastic Agents / pharmacology Apoptosis / drug effects* Cell Cycle Checkpoints / drug effects Cell Differentiation / drug effects* Cell Proliferation / drug effects Chromatography, High Pressure Liquid Humans K562 Cells Leukemia, Myelogenous, Chronic, BCR-ABL Positive / metabolism Leukemia, Myelogenous, Chronic, BCR-ABL Positive / pathology Monocytes / cytology Monocytes / drug effects Olea / chemistry* Olea / metabolism Plant Extracts / chemistry Plant Extracts / pharmacology* Plant Leaves / chemistry Plant Leaves / metabolism Transcriptome / drug effects
IF 4.936
Human and Animal Cells