論文 - 詳細
| RRC ID | 45062 |
|---|---|
| 著者 | Samet I, Han J, Jlaiel L, Sayadi S, Isoda H. |
| タイトル | Olive (Olea europaea) leaf extract induces apoptosis and monocyte/macrophage differentiation in human chronic myelogenous leukemia K562 cells: insight into the underlying mechanism. |
| ジャーナル | Oxid Med Cell Longev |
| Abstract |
Differentiation therapy is an attractive approach aiming at reversing malignancy and reactivating endogenous differentiation programs in cancer cells. Olive leaf extract, known for its antioxidant activity, has been demonstrated to induce apoptosis in several cancer cells. However, its differentiation inducing properties and the mechanisms involved are still poorly understood. In this study, we investigated the effect of Chemlali Olive Leaf Extract (COLE) for its potential differentiation inducing effect on multipotent leukemia K562 cells. Results showed that COLE inhibits K562 cells proliferation and arrests the cell cycle at G0/G1, and then at G2/M phase over treatment time. Further analysis revealed that COLE induces apoptosis and differentiation of K562 cells toward the monocyte lineage. Microarray analysis was conducted to investigate the underlying mechanism of COLE differentiation inducing effect. The differentially expressed genes such as IFI16, EGR1, NFYA, FOXP1, CXCL2, CXCL3, and CXCL8 confirmed the commitment of K562 cells to the monocyte/macrophage lineage. Thus our results provide evidence that, in addition to apoptosis, induction of differentiation is one of the possible therapeutic effects of olive leaf in cancer cells. |
| 巻・号 | 2014 |
| ページ | 927619 |
| 公開日 | 2014-1-1 |
| DOI | 10.1155/2014/927619 |
| PMID | 24803988 |
| PMC | PMC3997986 |
| MeSH | Antineoplastic Agents / pharmacology Apoptosis / drug effects* Cell Cycle Checkpoints / drug effects Cell Differentiation / drug effects* Cell Proliferation / drug effects Chromatography, High Pressure Liquid Humans K562 Cells Leukemia, Myelogenous, Chronic, BCR-ABL Positive / metabolism Leukemia, Myelogenous, Chronic, BCR-ABL Positive / pathology Monocytes / cytology Monocytes / drug effects Olea / chemistry* Olea / metabolism Plant Extracts / chemistry Plant Extracts / pharmacology* Plant Leaves / chemistry Plant Leaves / metabolism Transcriptome / drug effects |
| IF | 5.076 |
| 引用数 | 36 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | K562 |