| RRC ID |
45083
|
| Author |
Hosoki K, Kainuma K, Toda M, Harada E, Chelakkot-Govindalayathila AL, Roeen Z, Nagao M, D'Alessandro-Gabazza CN, Fujisawa T, Gabazza EC.
|
| Title |
Montelukast suppresses epithelial to mesenchymal transition of bronchial epithelial cells induced by eosinophils.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
Epithelial to mesenchymal transition (EMT) is a mechanism by which eosinophils can induce airway remodeling. Montelukast, an antagonist of the cysteinyl leukotriene receptor, can suppress airway remodeling in asthma. The purpose of this study was to evaluate whether montelukast can ameliorate airway remodeling by blocking EMT induced by eosinophils. EMT induced was assessed using a co-culture system of human bronchial epithelial cells and human eosinophils or the eosinophilic leukemia cell lines, Eol-1. Montelukast inhibited co-culture associated morphological changes of BEAS-2b cells, decreased the expression of vimentin and collagen I, and increased the expression of E-cadherin. Montelukast mitigated the rise of TGF-β1 production and Smad3 phosphorylation. Co-culture of human eosinophils with BEAS-2B cells significantly enhanced the production of CysLTs compared with BEAS-2B cells or eosinophils alone. The increase of CysLTs was abolished by montelukast pre-treatment. Montelukast had similar effects when co-culture system of Eol-1 and BEAS-2B was used. This study showed that montelukast suppresses eosinophils-induced EMT of airway epithelial cells. This finding may explain the mechanism of montelukast-mediated amelioration of airway remodeling in bronchial asthma.
|
| Volume |
449(3)
|
| Pages |
351-6
|
| Published |
2014-7-4
|
| DOI |
10.1016/j.bbrc.2014.05.033
|
| PII |
S0006-291X(14)00887-0
|
| PMID |
24845378
|
| MeSH |
Acetates / pharmacology*
Airway Remodeling / drug effects*
Asthma / metabolism
Asthma / pathology
Bronchi / cytology
Bronchi / drug effects*
Bronchi / metabolism
Cell Line, Tumor
Coculture Techniques
Collagen Type I / metabolism
Cyclopropanes
Cysteine / antagonists & inhibitors
Eosinophils / physiology
Epithelial-Mesenchymal Transition / drug effects*
Humans
Leukotriene Antagonists / pharmacology*
Leukotrienes
Phosphorylation
Quinolines / pharmacology*
Respiratory Mucosa / cytology
Respiratory Mucosa / drug effects*
Smad3 Protein / metabolism
Sulfides
Transforming Growth Factor beta1 / metabolism
Vimentin / metabolism
|
| IF |
2.985
|
| Times Cited |
19
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
EoL-1 cell(RCB0641) |
