Reference - Detail
| RRC ID | 45087 |
|---|---|
| Author | Hanaki K, Ike F, Kajita A, Yasuno W, Yanagiba M, Goto M, Sakai K, Ami Y, Kyuwa S. |
| Title | A broadly reactive one-step SYBR Green I real-time RT-PCR assay for rapid detection of murine norovirus. |
| Journal | PLoS One |
| Abstract |
A one-step SYBR Green I real-time RT-PCR assay was developed for the detection and quantification of a broad range of murine noroviruses (MNVs). The primer design was based on the multiple sequence alignments of 101 sequences of the open reading frame (ORF)1-ORF2 junction of MNV. The broad reactivity and quantitative capacity of the assay were validated using 7 MNV plasmids. The assay was completed within 1 h, and the reliable detection limit was 10 copies of MNV plasmid or 0.063 median tissue culture infective doses per milliliter of RAW264 cell culture-propagated viruses. The diagnostic performance of the assay was evaluated using 158 mouse fecal samples, 91 of which were confirmed to be positive. The melting curve analysis demonstrated the diversity of MNV in the samples. This is the first report of a broadly reactive one-step SYBR Green I real-time RT-PCR assay for detecting of MNVs. The rapid and sensitive performance of this assay makes it a powerful tool for diagnostic applications. |
| Volume | 9(5) |
| Pages | e98108 |
| Published | 2014-1-1 |
| DOI | 10.1371/journal.pone.0098108 |
| PII | PONE-D-13-44231 |
| PMID | 24847970 |
| PMC | PMC4029972 |
| MeSH | Animals Base Sequence Benzothiazoles Cell Line DNA Primers Diamines Feces / virology Mice Mice, Inbred C57BL Molecular Sequence Data Norovirus / genetics Norovirus / isolation & purification* Open Reading Frames Organic Chemicals / chemistry* Plasmids Quinolines RNA, Viral / genetics Real-Time Polymerase Chain Reaction / methods* Reproducibility of Results Temperature Time Factors |
| IF | 2.74 |
| Times Cited | 7 |
| WOS Category | VIROLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | RAW 264(RCB0535) |