| RRC ID |
45089
|
| 著者 |
Yamazaki T, Matsuo J, Nakamura S, Oguri S, Yamaguchi H.
|
| タイトル |
Effect of Ureaplasma parvum co-incubation on Chlamydia trachomatis maturation in human epithelial HeLa cells treated with interferon-γ.
|
| ジャーナル |
J Infect Chemother
|
| Abstract |
Chlamydia trachomatis is an obligate intracellular bacterium that causes a sexually transmitted disease. Ureaplasma parvum is commensal in the human genital tract, with a minimal contribution to urogenital infection. We have recently found that U. parvum has a significant effect on the presence of C. trachomatis in the genital tract of healthy women. We therefore assessed the effect of U. parvum co-incubation on C. trachomatis maturation from reticulate bodies (RBs) to elementary bodies (EBs) in HeLa cells in the absence or presence of interferon (IFN)-γ, which is a critical host defense factor. IFN-γ stimulation of viable U. parvum significantly prompted chlamydial growth with an increase in infectious particles, EBs, in HeLa cells. IFN-γ treatment of killed U. parvum had a similar effect on C. trachomatis maturation in HeLa cells. There was no change in expression of indoleamine 2,3-dioxygenase (IDO) in cultures of viable or killed U. parvum. We concluded that U. parvum co-incubation by IFN-γ helped C. trachomatis to mature from RBs to EBs in HeLa cells, independent of IDO expression. This suggests a novel survival strategy of C. trachomatis against IFN-γ exposure, prompting secondary infection of the genital mucosa, with possible clinical implications.
|
| 巻・号 |
20(8)
|
| ページ |
460-4
|
| 公開日 |
2014-8-1
|
| DOI |
10.1016/j.jiac.2014.04.003
|
| PII |
S1341-321X(14)00168-8
|
| PMID |
24855914
|
| MeSH |
Chlamydia Infections / microbiology*
Chlamydia trachomatis* / drug effects
Chlamydia trachomatis* / growth & development
Coculture Techniques
Coinfection / microbiology*
Female
HeLa Cells
Humans
Indoleamine-Pyrrole 2,3,-Dioxygenase / analysis
Indoleamine-Pyrrole 2,3,-Dioxygenase / metabolism
Interferon-gamma / pharmacology*
Models, Biological
Ureaplasma / drug effects*
Ureaplasma Infections / microbiology*
|
| IF |
1.722
|
| 引用数 |
7
|
|
WOS 分野
|
INFECTIOUS DISEASES
PHARMACOLOGY & PHARMACY
|
| リソース情報 |
| ヒト・動物細胞 |
HEp-2(RCB1889) |
