Reference - Detail
RRC ID | 45091 |
---|---|
Author | Trisonthi P, Sato A, Nishiwaki H, Tamura H. |
Title | A new diterpene from Litsea cubeba fruits: structure elucidation and capability to induce apoptosis in HeLa cells. |
Journal | Molecules |
Abstract |
A new diterpene, identified as (+)-6-(4-hydroxy-4-methyl-2-pentenoyl)-4,6-dimethyl-5-(3-methyl-2-butenyl)-1,3-cyclohexadienecarbaldehyde (1, cubelin), was isolated from a methanol extract of Litsea cubeba fruits by normal phase column chromatography and purified by preparative HPLC. The structure elucidation was conducted by spectroscopic methods (UV, IR, ESI-TOF-MS, 1-D and 2-D NMR). Cubelin exhibited activity against HeLa cell viability and proliferation. The cells also exhibited changes in nuclear morphology which are hallmarks of apoptotic cell death. The presence of cleaved caspase-3/-7, caspase-8 and caspase-9 in the cubelin treated population indicated the potential of the compound to induce apoptosis in HeLa cells via both intrinsic and extrinsic pathways. |
Volume | 19(5) |
Pages | 6838-50 |
Published | 2014-5-23 |
DOI | 10.3390/molecules19056838 |
PII | molecules19056838 |
PMID | 24858270 |
PMC | PMC6271781 |
MeSH | Antineoplastic Agents, Phytogenic / chemistry Antineoplastic Agents, Phytogenic / poisoning* Apoptosis / drug effects Caspase 3 / metabolism Caspase 8 / metabolism Caspase 9 / metabolism Cell Proliferation / drug effects Cell Survival / drug effects Diterpenes / chemistry* Diterpenes / pharmacology* Fruit / chemistry HeLa Cells / drug effects Humans Litsea / chemistry* Magnetic Resonance Spectroscopy Molecular Structure |
IF | 3.267 |
Times Cited | 5 |
WOS Category | CHEMISTRY, ORGANIC |
Resource | |
Human and Animal Cells | HeLa |