| RRC ID |
45096
|
| 著者 |
Mochizuki C, Hara H, Oya K, Aoki S, Hayakawa T, Fujie H, Sato M.
|
| タイトル |
Behaviors of MC3T3-E1 cells on carbonated apatite films, with a characteristic network structure, fabricated on a titanium plate by aqueous spray coating.
|
| ジャーナル |
Mater Sci Eng C Mater Biol Appl
|
| Abstract |
Four carbonated apatite films having average thicknesses of 1.3-0.11μm, proportions of network sizes above 10μm of 41-68%, and average border heights of the characteristic network structure of 0.98-0.29μm were fabricated on a titanium plate by aqueous spray coating. These carbonated apatite films after heat treatment showed good mineralization ability in Hanks' balanced salt solution. Assessment of initial cell attachment and calcination on these films and on the Ti plate using osteoblastic MC3T3-E1 indicated that the carbonated apatite film heat treated at 600°C, whose film thickness, proportion of network sizes above 10μm, and border height were 0.11μm, 61%, and 0.31μm, respectively, was most preferred by osteoblastic cells. Field emission scanning electron microscopic observation of the cells attached to the films showed that the wide network and low border height of the network structure on the carbonated apatite film play an important role in the development of the filopodia of the osteoblastic cells.
|
| 巻・号 |
39
|
| ページ |
245-52
|
| 公開日 |
2014-6-1
|
| DOI |
10.1016/j.msec.2014.02.040
|
| PII |
S0928-4931(14)00119-2
|
| PMID |
24863222
|
| MeSH |
3T3 Cells
Animals
Apatites / chemistry*
Cell Proliferation / drug effects
Mice
Microscopy, Electron, Scanning
Osteoblasts / cytology
Osteoblasts / drug effects
Osteoblasts / metabolism
Surface Properties
Titanium / chemistry*
|
| IF |
5.88
|
| 引用数 |
4
|
|
WOS 分野
|
MATERIALS SCIENCE, BIOMATERIALS
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126) |
