RRC ID |
45121
|
著者 |
Tomioka Y, Morimatsu M, Nishijima K, Usui T, Yamamoto S, Suyama H, Ozaki K, Ito T, Ono E.
|
タイトル |
A soluble form of Siglec-9 provides an antitumor benefit against mammary tumor cells expressing MUC1 in transgenic mice.
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ジャーナル |
Biochem Biophys Res Commun
|
Abstract |
Tumor-associated MUC1 binds to Siglec-9, which is expected to mediate tumor cell growth and negative immunomodulation. We hypothesized that a soluble form of Siglec-9 (sSiglec-9) competitively inhibits a binding of MUC1 to its receptor molecules like human Siglec-9, leading to provide antitumor benefit against MUC1-expressing tumor, and generated transgenic mouse lines expressing sSiglec-9 (sSiglec-9 Tg). When mammary tumor cells expressing MUC1 were intraperitoneally transplanted into sSiglec-9 Tg, tumor proliferation was slower with the lower histological malignancy as compared with non-transgenic mice. The sSiglec-9 was detected in the ascites caused by the tumor in the sSiglec-9 Tg, and sSiglec-9 and MUC1 were often colocalized on surfaces of the tumor cells. PCNA immunohistochemistry also revealed the reduced proliferation of the tumor cells in sSiglec-9 Tg. In sSiglec-9 Tg with remarkable suppression of tumor proliferation, MUC1 expressions were tend to be reduced. In the ascites of sSiglec-9 Tg bearing the tumor, T cells were uniformly infiltrated, whereas aggregations of degenerative T cells were often observed in the non-transgenic mice. These results suggest that sSiglec-9 has an antitumor benefit against MUC1-expressing tumor in the transgenic mice, which may avoid the negative immunomodulation and/or suppress tumor-associated MUC1 downstream signal transduction, and subsequent tumor proliferation.
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巻・号 |
450(1)
|
ページ |
532-7
|
公開日 |
2014-7-18
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DOI |
10.1016/j.bbrc.2014.06.009
|
PII |
S0006-291X(14)01061-4
|
PMID |
24924635
|
MeSH |
Animals
Antigens, CD / chemistry
Antigens, CD / genetics
Antigens, CD / metabolism*
Antigens, Differentiation, B-Lymphocyte / chemistry
Antigens, Differentiation, B-Lymphocyte / genetics
Antigens, Differentiation, B-Lymphocyte / metabolism*
Cell Line, Tumor
Cell Survival
Female
Humans
Mammary Neoplasms, Animal / metabolism*
Mammary Neoplasms, Animal / pathology
Mammary Neoplasms, Animal / therapy*
Mice
Mice, Inbred C57BL
Mice, Transgenic
Mucin-1 / genetics
Mucin-1 / metabolism*
Solubility
|
IF |
2.985
|
引用数 |
10
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
|