| RRC ID |
45140
|
| Author |
Uraki S, Sugimoto K, Shiraki K, Tameda M, Inagaki Y, Ogura S, Kasai C, Nojiri K, Yoneda M, Yamamoto N, Takei Y, Nobori T, Ito M.
|
| Title |
Human β-defensin-3 inhibits migration of colon cancer cells via downregulation of metastasis-associated 1 family, member 2 expression.
|
| Journal |
Int J Oncol
|
| Abstract |
The innate immune system plays an important role as the first line of defense against many types of microbes. Accumulating reports suggest that human β-defensins (hBDs) are expressed by and have certain roles in some cancer cells. In this study, we investigated the roles of hBD-3 in colon cancer cells. The expression of hBD-3 was examined by reverse transcriptase-polymerase chain reaction analysis of colon cancer cell lines and immunohistochemical staining of colon cancer tissues. The effect of hBD-3 on proliferation of colon cancer was assessed using the MTT assay and a real-time cell analyzer, and the effect of hBD-3 on the migration of colon cancer cells was also examined. The results showed that hBD-3 is not expressed in colon cancer cells but is produced by tumor-infiltrating monocytes. Migration of colon cancer cells was significantly inhibited by hBD-3 in a dose-dependent manner, although proliferation of colon cancer cells was not affected by administration of hBD-3. Moreover, reduced expression of metastasis-associated 1 family, member 2 (MTA2) mRNA in colon cancer cells was associated with exposure to hBD-3. In conclusion, progression of colon cancer was inhibited by hBD-3 in a paracrine fashion. Therefore, hBD-3 may be a potent new agent for treating colon cancer.
|
| Volume |
45(3)
|
| Pages |
1059-64
|
| Published |
2014-9-1
|
| DOI |
10.3892/ijo.2014.2507
|
| PMID |
24969834
|
| MeSH |
Cell Line, Tumor
Cell Proliferation
Colonic Neoplasms / genetics
Colonic Neoplasms / pathology*
Gene Expression Regulation, Neoplastic
HT29 Cells
Histone Deacetylases / genetics*
Humans
Neoplasm Invasiveness / genetics*
Paracrine Communication
RNA, Messenger / genetics
Repressor Proteins / genetics*
beta-Defensins / genetics*
beta-Defensins / metabolism*
|
| IF |
3.899
|
| Times Cited |
19
|
|
WOS Category
|
ONCOLOGY
|
| Resource |
| Human and Animal Cells |
COLO-320(RCB1193) |
