論文 - 詳細
| RRC ID | 45141 |
|---|---|
| 著者 | Yu Y, Yang D, Qiu L, Okamura H, Guo J, Haneji T. |
| タイトル | Tumor necrosis factor-α induces interleukin-34 expression through nuclear factor‑κB activation in MC3T3-E1 osteoblastic cells. |
| ジャーナル | Mol Med Rep |
| Abstract |
Osteoblasts produce various types of cytokines under pathological conditions and control osteoclast differentiation. Tumor necrosis factor-α (TNF-α) has been demonstrated to exert complex effects in osteoblasts under local inflammatory conditions, including in periodontal and periapical diseases. Interleukin-34 (IL-34) has been recently identified as a novel regulatory factor for the differentiation and function of osteoclasts. The present study provides the first evidence, to the best of our knowledge, that the expression of IL-34 is induced by TNF-α through nuclear factor-κB (NF-κB) activation in MC3T3-E1 osteoblastic cells. TNF-α induced IL-34 expression in a dose- and time-dependent manner. Immunocytochemistry with an NF-κB antibody demonstrated that NF-κB was mainly localized in the cytoplasm of the untreated MC3T3-E1 cells. Rapid translocation of NF-κB from the cytoplasm to the nucleus was observed in the cells treated with TNF-α for 15 min. Translocation and transcriptional activity of NF-κB were also determined by western blotting and a luciferase reporter assay, respectively. Pretreatment with 100 µM CAPE, an inhibitor of NF-κB, significantly inhibited TNF-α-induced IL-34 expression. These results indicate that TNF-α induces IL-34 expression via NF-κB in osteoblasts. |
| 巻・号 | 10(3) |
| ページ | 1371-6 |
| 公開日 | 2014-9-1 |
| DOI | 10.3892/mmr.2014.2353 |
| PMID | 24970360 |
| PMC | PMC4121411 |
| MeSH | 3T3 Cells Animals Cell Differentiation / drug effects Cell Nucleus / drug effects Cell Nucleus / metabolism Dose-Response Relationship, Drug Interleukins / genetics Interleukins / metabolism* Mice NF-kappa B / genetics NF-kappa B / metabolism* Osteoblasts / cytology Osteoblasts / drug effects* Osteoblasts / metabolism Signal Transduction Tumor Necrosis Factor-alpha / pharmacology* |
| IF | 2.1 |
| 引用数 | 22 |
| WOS 分野 | MEDICINE, RESEARCH & EXPERIMENTAL ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |