| RRC ID |
45141
|
| 著者 |
Yu Y, Yang D, Qiu L, Okamura H, Guo J, Haneji T.
|
| タイトル |
Tumor necrosis factor-α induces interleukin-34 expression through nuclear factor‑κB activation in MC3T3-E1 osteoblastic cells.
|
| ジャーナル |
Mol Med Rep
|
| Abstract |
Osteoblasts produce various types of cytokines under pathological conditions and control osteoclast differentiation. Tumor necrosis factor-α (TNF-α) has been demonstrated to exert complex effects in osteoblasts under local inflammatory conditions, including in periodontal and periapical diseases. Interleukin-34 (IL-34) has been recently identified as a novel regulatory factor for the differentiation and function of osteoclasts. The present study provides the first evidence, to the best of our knowledge, that the expression of IL-34 is induced by TNF-α through nuclear factor-κB (NF-κB) activation in MC3T3-E1 osteoblastic cells. TNF-α induced IL-34 expression in a dose- and time-dependent manner. Immunocytochemistry with an NF-κB antibody demonstrated that NF-κB was mainly localized in the cytoplasm of the untreated MC3T3-E1 cells. Rapid translocation of NF-κB from the cytoplasm to the nucleus was observed in the cells treated with TNF-α for 15 min. Translocation and transcriptional activity of NF-κB were also determined by western blotting and a luciferase reporter assay, respectively. Pretreatment with 100 µM CAPE, an inhibitor of NF-κB, significantly inhibited TNF-α-induced IL-34 expression. These results indicate that TNF-α induces IL-34 expression via NF-κB in osteoblasts.
|
| 巻・号 |
10(3)
|
| ページ |
1371-6
|
| 公開日 |
2014-9-1
|
| DOI |
10.3892/mmr.2014.2353
|
| PMID |
24970360
|
| PMC |
PMC4121411
|
| MeSH |
3T3 Cells
Animals
Cell Differentiation / drug effects
Cell Nucleus / drug effects
Cell Nucleus / metabolism
Dose-Response Relationship, Drug
Interleukins / genetics
Interleukins / metabolism*
Mice
NF-kappa B / genetics
NF-kappa B / metabolism*
Osteoblasts / cytology
Osteoblasts / drug effects*
Osteoblasts / metabolism
Signal Transduction
Tumor Necrosis Factor-alpha / pharmacology*
|
| IF |
2.1
|
| 引用数 |
22
|
|
WOS 分野
|
MEDICINE, RESEARCH & EXPERIMENTAL
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126) |
