RRC ID |
45145
|
Author |
Yokooji T, Kameda Y, Utsumi M, Mori N, Murakami T.
|
Title |
Interaction of hydrophobic components in female urine before and after childbirth with P-glycoprotein in vitro.
|
Journal |
Pharmazie
|
Abstract |
The first urine in the morning (total 15 samples) and whole day urine (total 4 days, 17 samples) were collected from a young healthy woman during the pregnancy and lactation period, to examine the possible interactions of urine components (methanol extracts) with P-glycoprotein (P-gp) and multidrug resistance-associated proteins (MRPs). The interaction was evaluated by measuring the intracellular accumulation of rhodamine123, a P-gp substrate, in LLC-GA5-COL150 cells, or calcein, an MRP substrate, in Caco-2 cells in the absence and presence of urine components. Four first urine samples out of 12 collected before childbirth and one sample out of three collected after childbirth suppressed P-gp function significantly. The effect of pregnancy and lactation on P-gp inhibitory potencies of urine components was not observed. The whole day urine samples showed a clear circadian rhythm, in which three first urine samples in the morning out of four showed greater P-gp inhibitory potencies than other daytime samples. Interaction of urine components with MRPs was not detected. In conclusion, the concentration of endogenous P-gp inhibitor(s) was higher in the first urine in the morning, showing a clear circadian rhythm. Normal pregnancy and lactation appeared not to significantly affect the P-gp inhibitory potencies of urine components.
|
Volume |
69(6)
|
Pages |
430-6
|
Published |
2014-6-1
|
PMID |
24974576
|
MeSH |
ATP Binding Cassette Transporter, Subfamily B / genetics
ATP Binding Cassette Transporter, Subfamily B / metabolism
ATP Binding Cassette Transporter, Subfamily B, Member 1 / urine*
ATP-Binding Cassette Transporters / biosynthesis
Adult
Blotting, Western
Caco-2 Cells
Cell Line
Circadian Rhythm
Female
Fluoresceins / metabolism
Fluorescent Dyes
Humans
Lactation / physiology
Postpartum Period / urine*
Pregnancy
Rhodamine 123
Young Adult
|
IF |
1.198
|
Times Cited |
2
|
WOS Category
|
PHARMACOLOGY & PHARMACY
CHEMISTRY, MULTIDISCIPLINARY
CHEMISTRY, MEDICINAL
|
Resource |
Human and Animal Cells |
CACO-2(RCB0988) |