論文 - 詳細
| RRC ID | 45153 |
|---|---|
| 著者 | Takeda A, Tsubaki T, Sagae N, Onda Y, Inada Y, Mochizuki T, Okumura K, Kikuyama S, Kobayashi T, Iwamuro S. |
| タイトル | Bacterial toxin-inducible gene expression of cathelicidin-B1 in the chicken bursal lymphoma-derived cell line DT40: functional characterization of cathelicidin-B1. |
| ジャーナル | Peptides |
| Abstract |
Chicken cathelicidin-B1 (chCATH-B1) is a major host defense peptide of the chicken bursa of Fabricius (BF). To investigate the mechanisms of chCATH-B1 gene expression in the BF, we focused on the DT40 cell line derived from chicken bursal lymphoma as a model for analysis. A cDNA encoding chCATH-B1 precursor was cloned from DT40 cells. The nucleotide sequence of the cDNA was identical with that of the BF chCATH-B1. A broth dilution analysis showed that the synthetic chCATH-B1 exhibited a significant defensive activity against both Escherichia coli and Staphylococcus aureus. A scanning microscopic analysis demonstrated that chCATH-B1 inhibited bacterial growth through membrane destruction with formation of blebs and spheroplasts. Limulus amoebocyte lysate assay and electromobility shift assay results revealed that chCATH-B1 bound to lipopolysaccharide (LPS) and lipoteichoic acid (LTA), which are the surface substances of the E. coli and S. aureus cell, respectively. A chemotactic assay results revealed that chCATH-B1 showed mouse-derived P-815 mastocytoma migrating activity dose-dependently but with a higher concentration, resulting in a loss of the activity. A semi-quantitative real-time RT-PCR analysis revealed that LPS stimulated chCATH-B1 gene expression in a dose-dependent manner and that the LPS-inducible chCATH-B1 gene expression was inhibited by the administration of dexamethasone. The chCATH-B1 mRNA levels in DT40 cells were also increased by the administration of bacterial LTA. The results indicate that bacterial toxins induce chCATH-B1 gene expression in the chicken BF and the peptide expressed in the organ would act against pathogenic microorganisms not only directly but also indirectly by attracting mast cells. |
| 巻・号 | 59 |
| ページ | 94-102 |
| 公開日 | 2014-9-1 |
| DOI | 10.1016/j.peptides.2014.06.012 |
| PII | S0196-9781(14)00185-5 |
| PMID | 24984089 |
| MeSH | Animals Antimicrobial Cationic Peptides / genetics* Antimicrobial Cationic Peptides / metabolism Antimicrobial Cationic Peptides / pharmacology* Bacterial Toxins / chemistry Bacterial Toxins / pharmacology* Cell Line Chickens Cloning, Molecular Dose-Response Relationship, Drug Escherichia coli / drug effects Gene Expression Regulation / drug effects* Gene Expression Regulation / genetics Lymphoma / genetics* Lymphoma / metabolism Microbial Sensitivity Tests RNA, Messenger / drug effects RNA, Messenger / genetics Staphylococcus aureus / drug effects Structure-Activity Relationship |
| IF | 2.843 |
| 引用数 | 1 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY ENDOCRINOLOGY & METABOLISM PHARMACOLOGY & PHARMACY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | P-815(RCB1167) COS-7(RCB0539) |