Reference - Detail
| RRC ID | 45154 |
|---|---|
| Author | Aizawa T, Kim SY, Takahashi S, Koshita M, Tani M, Futamura Y, Osada H, Funa N. |
| Title | Alkyldihydropyrones, new polyketides synthesized by a type III polyketide synthase from Streptomyces reveromyceticus. |
| Journal | J Antibiot (Tokyo) |
| Abstract |
Genome sequencing allows a rapid and efficient identification of novel catalysts that produce novel secondary metabolites. Here we describe the catalytic properties of dihydropyrone synthase A (DpyA), a novel type III polyketide synthase encoded in a linear plasmid of Streptomyces reveromyceticus. Heterologous expression of dpyA led to the accumulation of alkyldihydropyrones A (1), B (2), C (3) and D (4), which are novel dihydropyran compounds that exhibit weak cytotoxicity against the leukemia cell line HL-60. DpyA catalyzes the condensation of β-hydroxyl acid thioester and methylmalonyl-CoA to yield a triketide intermediate that then undergoes lactonization of a secondary alcohol and a thioester to give alkyldihydropyrone. |
| Volume | 67(12) |
| Pages | 819-23 |
| Published | 2014-12-1 |
| DOI | 10.1038/ja.2014.80 |
| PII | ja201480 |
| PMID | 24984800 |
| MeSH | Antineoplastic Agents / chemistry Antineoplastic Agents / metabolism* Antineoplastic Agents / pharmacology Cell Survival / drug effects HL-60 Cells Humans Kinetics Magnetic Resonance Spectroscopy Molecular Structure Optical Rotation Plasmids / genetics Polyketide Synthases / genetics Polyketide Synthases / metabolism* Polyketides / chemical synthesis* Polyketides / chemistry Polyketides / metabolism Polyketides / pharmacology Pyrones / chemical synthesis* Pyrones / chemistry Pyrones / pharmacology Spectrophotometry, Ultraviolet Spectroscopy, Fourier Transform Infrared Streptomyces / enzymology* Streptomyces / genetics |
| IF | 2.668 |
| Times Cited | 5 |
| WOS Category | BIOTECHNOLOGY & APPLIED MICROBIOLOGY PHARMACOLOGY & PHARMACY IMMUNOLOGY MICROBIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| Total number of mentions | 0 |
| Resource | |
| Human and Animal Cells | HL-60 |