論文 - 詳細
| RRC ID | 45161 |
|---|---|
| 著者 | Kitamura N, Kurokawa T, Fukui T, Gong JP, Yasuda K. |
| タイトル | Hyaluronic acid enhances the effect of the PAMPS/PDMAAm double-network hydrogel on chondrogenic differentiation of ATDC5 cells. |
| ジャーナル | BMC Musculoskelet Disord |
| Abstract |
BACKGROUND:A double-network (DN) gel, which was composed of poly-(2-Acrylamido-2-methylpropanesulfonic acid) and poly-(N,N'-dimethyl acrylamide) (PAMPS/PDMAAm), has the potential to induce chondrogenesis both in vitro and in vivo. The present study investigated whether DN gel induced chondrogenic differentiation of ATDC5 cells in a maintenance medium without insulin, and whether supplementation of hyaluronic acid enhanced the chondrogenic differentiation effect of DN gel. METHODS:ATDC5 cells were cultured on the DN gel and the polystyrene (PS) dish in maintenance media without insulin for 21 days. Hyaluronic acid having a molecular weight of approximately 800 kDa was supplemented into the medium so that the concentration became 0.01, 0.1, or 1.0 mg/mL. The cultured cells were evaluated using immunocytochemistry for type-2 collagen and real time PCR for gene expression of type-2 collagen, aggrecan, and Sox9 at 7 and 21 days of culture. RESULTS:The cells cultured on the DN gel formed nodules and were stained with an anti-type-2 collagen antibody, and expression of type-2 collagen and aggrecan mRNA was significantly greater on the DN gel than on the PS dish surface (p < 0.05) in the hyaluronic acid-free maintenance medium. Hyaluronic acid supplementation of a high concentration (1.0 mg/mL) significantly enhanced expression of type-2 collagen and aggrecan mRNA in comparison with culture without hyaluronic acid at 21 days (p < 0.05). CONCLUSIONS:The DN gel induced chondrogenic differentiation of ATDC5 cells without insulin. This effect was significantly affected by hyaluronic acid, depending on the level of concentration. There is a high possibility that hyaluronic acid plays an important role in the in vivo hyaline cartilage regeneration phenomenon induced by the DN gel. |
| 巻・号 | 15 |
| ページ | 222 |
| 公開日 | 2014-7-6 |
| DOI | 10.1186/1471-2474-15-222 |
| PII | 1471-2474-15-222 |
| PMID | 24997593 |
| PMC | PMC4107725 |
| MeSH | Acrylamides / pharmacology* Aggrecans / genetics Aggrecans / metabolism Animals Cell Culture Techniques Cell Differentiation / drug effects* Cell Line Chondrocytes / drug effects* Chondrocytes / metabolism Chondrogenesis / drug effects* Collagen Type II / genetics Collagen Type II / metabolism Dose-Response Relationship, Drug Hyaluronic Acid / pharmacology* Hydrogels Mice Polymers / pharmacology* RNA, Messenger / metabolism SOX9 Transcription Factor / genetics SOX9 Transcription Factor / metabolism Sulfonic Acids / pharmacology* Time Factors |
| IF | 1.879 |
| 引用数 | 10 |
| WOS 分野 | ORTHOPEDICS RHEUMATOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | ATDC5(RCB0565) |