Reference - Detail
RRC ID | 45181 |
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Author | Gamo K, Miyachi H, Nakamura K, Matsuura N. |
Title | Hesperetin glucuronides induce adipocyte differentiation via activation and expression of peroxisome proliferator-activated receptor-γ. |
Journal | Biosci Biotechnol Biochem |
Abstract |
In previous reports, hesperidin, a flavonoid glucoside from citrus fruit, is hydrolyzed to hesperetin, an aglycone of hesperidin, and converted to the hesperetin glucuronides (H7-OG and H3'-OG) in vivo and depresses blood glucose levels. But there are no reports on the activity of hesperetin glucuronides. To determine the activity of hesperetin glucuronides, H7-OG and H3'-OG were synthesized and peroxisome proliferator-activated receptor-γ (PPARγ) agonist activity was observed at 250 μM. These glucuronides accelerated the differentiation of 3T3-L1 cells into adipocytes at 10 μM. Furthermore, H7-OG showed additive effects in reporter gene assays and caused noncompetitive reactions in time-resolved fluorescence resonance energy transfer assays with a thiazolidinedione derivative. Our results indicated that hesperetin glucuronides activated PPARγ, accelerated adipocyte differentiation. |
Volume | 78(6) |
Pages | 1052-9 |
Published | 2014-1-1 |
DOI | 10.1080/09168451.2014.910097 |
PMID | 25036134 |
MeSH | 3T3-L1 Cells Adipocytes / cytology* Adipocytes / drug effects Animals COS Cells Cell Differentiation / drug effects* Chlorocebus aethiops Chromans / pharmacology Drug Synergism Gene Expression Regulation / drug effects* Glucuronides / chemistry* Hesperidin / chemistry* Hesperidin / metabolism Hesperidin / pharmacology* Mice PPAR gamma / agonists PPAR gamma / chemistry PPAR gamma / genetics PPAR gamma / metabolism* Protein Structure, Tertiary Thiazolidinediones / pharmacology Troglitazone |
IF | 1.516 |
Times Cited | 6 |
WOS Category | FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY CHEMISTRY, APPLIED BIOCHEMISTRY & MOLECULAR BIOLOGY |
Resource | |
Human and Animal Cells | COS-1(RCB0143) |