| RRC ID |
45182
|
| 著者 |
Nishiura H, Yamanegi K, Kawabe M, Kato-Kogoe N, Yamada N, Nakasho K.
|
| タイトル |
Annexin A3 plays a role in cytoplasmic calcium oscillation by extracellular calcium in the human promyelocytic leukemia HL-60 cells differentiated by phorbol-12-myristate-13-acetate.
|
| ジャーナル |
Exp Mol Pathol
|
| Abstract |
The roles of annexin A3 (ANXA3) in macrophages are not fully understood. In contrast to C5a, we have demonstrated that C-terminal ribosomal protein S19 (RP S19)-tagged S-tagged C5a (S-tagged C5a/RP S19) raises an alternative cytoplasmic calcium oscillation by extracellular calcium during macrophage migration into apoptotic cells. We here differentiated human promyelocytic leukemia HL-60 cells bearing with either control sense RNA and shRNA for ANXA3 mRNA or a vector cDNA with or without ANXA3 cDNA into macrophage-like cells by phorbol-12-myristate-13-acetate and found that a fluorescence ratio (340 nm/380 nm) upon the S-tagged C5a/RP S19-induced alternative cytoplasmic calcium oscillation by extracellular calcium was an equilateral association with a dose of ANXA3. Moreover, the ANXA3-dependent modification was partially reflected upon the S-tagged C5a-induced classical cytoplasmic calcium oscillation by both intracellular calcium and extracellular calcium. ANXA3 seems to extend the C5aR-mediated cytoplasmic calcium oscillation by extracellular calcium at least in the HL-60 macrophage-like cells.
|
| 巻・号 |
97(2)
|
| ページ |
241-6
|
| 公開日 |
2014-10-1
|
| DOI |
10.1016/j.yexmp.2014.07.010
|
| PII |
S0014-4800(14)00107-5
|
| PMID |
25036403
|
| MeSH |
Annexin A3 / genetics
Annexin A3 / metabolism*
Calcium / pharmacology
Calcium Signaling*
Cell Differentiation
HL-60 Cells
Humans
Macrophages / drug effects
Macrophages / metabolism
Receptor, Anaphylatoxin C5a / genetics
Receptor, Anaphylatoxin C5a / metabolism
Tetradecanoylphorbol Acetate / pharmacology
|
| IF |
2.28
|
| 引用数 |
2
|
|
WOS 分野
|
PATHOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
HL60 |
