論文 - 詳細
| RRC ID | 45187 |
|---|---|
| 著者 | Kanayama I, Miyaji H, Takita H, Nishida E, Tsuji M, Fugetsu B, Sun L, Inoue K, Ibara A, Akasaka T, Sugaya T, Kawanami M. |
| タイトル | Comparative study of bioactivity of collagen scaffolds coated with graphene oxide and reduced graphene oxide. |
| ジャーナル | Int J Nanomedicine |
| Abstract |
BACKGROUND:Graphene oxide (GO) is a single layer carbon sheet with a thickness of less than 1 nm. GO has good dispersibility due to surface modifications with numerous functional groups. Reduced graphene oxide (RGO) is produced via the reduction of GO, and has lower dispersibility. We examined the bioactivity of GO and RGO films, and collagen scaffolds coated with GO and RGO. METHODS:GO and RGO films were fabricated on a culture dish. Some GO films were chemically reduced using either ascorbic acid or sodium hydrosulfite solution, resulting in preparation of RGO films. The biological properties of each film were evaluated by scanning electron microscopy (SEM), atomic force microscopy, calcium adsorption tests, and MC3T3-E1 cell seeding. Subsequently, GO- and RGO-coated collagen scaffolds were prepared and characterized by SEM and compression tests. Each scaffold was implanted into subcutaneous tissue on the backs of rats. Measurements of DNA content and cell ingrowth areas of implanted scaffolds were performed 10 days post-surgery. RESULTS:The results show that GO and RGO possess different biological properties. Calcium adsorption and alkaline phosphatase activity were strongly enhanced by RGO, suggesting that RGO is effective for osteogenic differentiation. SEM showed that RGO-modified collagen scaffolds have rough, irregular surfaces. The compressive strengths of GO- and RGO-coated scaffolds were approximately 1.7-fold and 2.7-fold greater, respectively, when compared with the non-coated scaffold. Tissue ingrowth rate was 39% in RGO-coated scaffolds, as compared to 20% in the GO-coated scaffold and 16% in the non-coated scaffold. CONCLUSION:In summary, these results suggest that GO and RGO coatings provide different biological properties to collagen scaffolds, and that RGO-coated scaffolds are more bioactive than GO-coated scaffolds. |
| 巻・号 | 9 |
| ページ | 3363-73 |
| 公開日 | 2014-1-1 |
| DOI | 10.2147/IJN.S62342 |
| PII | ijn-9-3363 |
| PMID | 25050063 |
| PMC | PMC4103921 |
| MeSH | Alkaline Phosphatase / metabolism Animals Back / surgery Biocompatible Materials / chemistry* Biocompatible Materials / pharmacology Calcium / metabolism Cell Line Cell Proliferation / drug effects Collagen / chemistry* Collagen / pharmacology DNA Graphite / chemistry* Graphite / pharmacology Male Mice Prostheses and Implants Rats Rats, Wistar Tissue Engineering Tissue Scaffolds / chemistry* |
| IF | 5.115 |
| 引用数 | 52 |
| WOS 分野 | PHARMACOLOGY & PHARMACY NANOSCIENCE & NANOTECHNOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |