論文 - 詳細
| RRC ID | 45198 |
|---|---|
| 著者 | Ozaki Y, Takagi Y, Mori H, Hara M. |
| タイトル | Porous hydrogel of wool keratin prepared by a novel method: an extraction with guanidine/2-mercaptoethanol solution followed by a dialysis. |
| ジャーナル | Mater Sci Eng C Mater Biol Appl |
| Abstract |
In this study, we show a novel simple method to prepare a sponge-like porous keratin hydrogel through the extraction of wool keratin in a solution containing guanidine hydrochloride and 2-mercaptoethanol followed by dialysis for both aggregation of keratin and recrosslink. The gel had a highly porous structure and a fast-swelling property in rehydration after freeze-drying. It had also high mechanical strength both in the tensile test and the measurement of dynamic viscoelasticity. Three types of animal cells, PC12 cells, HOS cells and murine embryonic fibroblasts, well attached and grew on the surface of the porous hydrogel. |
| 巻・号 | 42 |
| ページ | 146-54 |
| 公開日 | 2014-9-1 |
| DOI | 10.1016/j.msec.2014.05.018 |
| PII | S0928-4931(14)00294-X |
| PMID | 25063104 |
| MeSH | Animals Biocompatible Materials / chemistry* Biocompatible Materials / isolation & purification* Cell Adhesion Cell Culture Techniques / instrumentation Cell Line Cell Survival Dialysis Guanidine Humans Hydrogel, Polyethylene Glycol Dimethacrylate / chemistry* Keratins / chemistry* Keratins / isolation & purification* Mercaptoethanol Mice PC12 Cells Porosity Rats Sheep Tissue Scaffolds / chemistry Wool / chemistry* |
| IF | 5.88 |
| 引用数 | 15 |
| WOS 分野 | MATERIALS SCIENCE, BIOMATERIALS |
| リソース情報 | |
| ヒト・動物細胞 | PC-12(RCB0009) HOS(RCB0992) |