RRC ID 45200
著者 Takebe J, Miyata K, Miura S, Ito S.
タイトル Effects of the nanotopographic surface structure of commercially pure titanium following anodization-hydrothermal treatment on gene expression and adhesion in gingival epithelial cells.
ジャーナル Mater Sci Eng C Mater Biol Appl
Abstract The long-term stability and maintenance of endosseous implants with anodized-hydrothermally treated commercially pure titanium surfaces and a nanotopographic structure (SA-treated c.p.Ti) depend on the barrier function provided by the interface between the transmucosal portion of the implant surface and the peri-implant epithelium. This study investigated the effects of extracellular and intracellular gene expression in adherent gingival epithelial cells cultured for 1-7 days on SA-treated c.p.Ti implant surfaces compared to anodic oxide (AO) c.p.Ti and c.p.Ti disks. Scanning electron microscopy (SEM) showed filopodium-like extensions bound closely to the nanotopographic structure of SA-treated c.p.Ti at day 7 of culture. Gene expressions of focal adhesion kinase, integrin-α6β4, and laminin-5 (α3, β3, γ2) were significantly higher on SA-treated c.p.Ti than on c.p.Ti or AO c.p.Ti after 7 days (P<0.05). Our results confirmed that gingival epithelial cells adhere to SA-treated c.p.Ti as the transmucosal portion of an implant, and that this interaction markedly improves expression of focal adhesion molecules and enhances the epithelial cell phenotype. The cellular gene expression responses driving extracellular and intracellular molecular interactions thus play an important role in maintenance at the interface between SA-treated c.p.Ti implant surfaces and the gingival epithelial cells.
巻・号 42
ページ 273-9
公開日 2014-9-1
DOI 10.1016/j.msec.2014.05.040
PII S0928-4931(14)00316-6
PMID 25063119
MeSH Cell Adhesion / drug effects* Cell Adhesion Molecules / analysis Cell Adhesion Molecules / genetics Cell Adhesion Molecules / metabolism Cell Line Cell Proliferation / drug effects Cell Shape / drug effects Electrodes Epithelial Cells / drug effects Gene Expression / drug effects* Integrin alpha6beta4 / analysis Integrin alpha6beta4 / genetics Integrin alpha6beta4 / metabolism Nanostructures / chemistry Nanostructures / ultrastructure* Nanotechnology Real-Time Polymerase Chain Reaction Surface Properties Titanium / chemistry* Titanium / pharmacology
IF 5.88
引用数 2
WOS 分野 MATERIALS SCIENCE, BIOMATERIALS
リソース情報
ヒト・動物細胞 GE1(RCB1709)