論文 - 詳細
| RRC ID | 45208 |
|---|---|
| 著者 | Suzuki R, Matsuno S, Sakagami H, Okada Y, Shirataki Y. |
| タイトル | Search of new cytotoxic crude materials against human oral squamous cell carcinoma using 1H NMR-based metabolomics. |
| ジャーナル | Anticancer Res |
| Abstract |
BACKGROUND:The 5-year survival rate of the oral cancer patients has remained at approximately the 50% level during the past 30 years, possibly due to the poor tumor-selectivity of conventional anticancer drugs. This prompted us to search new plant extracts that have higher cytotoxicity against cancer cells than normal cells. MATERIALS AND METHODS:Two human oral squamous cell carcinoma cell lines (HSC-2 and HSC-4) and two normal oral cells (gingival and periodontal ligament fibroblasts; HGF and HPLF) were incubated for 48 h with various concentrations of crude plant extract and the viable cell number was determined by the (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method. The 50% cytotoxic concentration (CC50) was determined from the dose-response curve. Tumor-specificity (TS) was determined by the following equation: TS=mean CC50 (normal cells)/mean CC50 (cancer cell lines). Metabolic profiling techniques based on (1)H nuclear magnetic resonance (NMR) were applied to gain the chemical structural insight for cytotoxicity induction. RESULTS:Among 24 plant extracts, Camptotheca acuminate leaf, a well-known source for camptothecin, showed the highest TS value (88.3), followed by Vitis s.p.p. (>3.5), Sasa veitchii (>2.3) and Phellodendron amurense (>2.1), whereas other plant extracts showed much lower TS value (<2). These cytotoxic extracts made cluster on principal component analysis (PCA) score plot. CONCLUSION:The TS value determined by the present method seems to reflect the anti-tumor potential of each plant extract, while a part of the cytotoxic compounds present in these extracts may have common chemical structures. |
| 巻・号 | 34(8) |
| ページ | 4117-20 |
| 公開日 | 2014-8-1 |
| PII | 34/8/4117 |
| PMID | 25075037 |
| MeSH | Antineoplastic Agents, Phytogenic / pharmacology* Carcinoma, Squamous Cell / drug therapy* Carcinoma, Squamous Cell / pathology Cell Line, Tumor Humans Magnetic Resonance Spectroscopy / methods* Metabolomics* Mouth Neoplasms / drug therapy* Mouth Neoplasms / pathology Plant Extracts / pharmacology* |
| IF | 1.994 |
| 引用数 | 20 |
| WOS 分野 | ONCOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | HSC-2(RCB1945) HSC-4(RCB1902) |