Reference - Detail
| RRC ID | 45227 |
|---|---|
| Author | Yamashita K, Ohkura T. |
| Title | Determination of glycan motifs using serial lectin affinity chromatography. |
| Journal | Methods Mol Biol |
| Abstract |
Serial lectin affinity chromatography is a convenient technique for characterizing glycan motifs (terminal glycan structures) of glycoproteins or released glycans. When these glycoconjugates are applied serially or in parallel to lectin-immobilized columns, information regarding the glycan motifs can be obtained. We demonstrate lectin affinity chromatographic methods for determining O-linked glycan structures of MUC1 purified from a breast cancer cell line, YMB-S, N-linked glycan structures of serum prostate-specific antigen from prostate cancer, and serum alkaline phosphatases from choriocarcinoma. These lectin-fractionated samples are analyzed quantitatively by measuring radioactivity, antigen contents are analyzed using enzyme-linked immunosorbent assay, and enzymatic activities are assessed. |
| Volume | 1200 |
| Pages | 79-92 |
| Published | 2014-1-1 |
| DOI | 10.1007/978-1-4939-1292-6_7 |
| PMID | 25117226 |
| MeSH | Cell Line, Tumor Chromatography, Affinity / methods* Glycoside Hydrolases / metabolism Humans Immobilized Proteins / chemistry Immobilized Proteins / metabolism Lectins / chemistry Lectins / metabolism* Male Mucin-1 / blood Mucin-1 / chemistry Mucin-1 / isolation & purification Mucin-1 / metabolism Polysaccharides / chemistry* Polysaccharides / isolation & purification Polysaccharides / metabolism Prostatic Hyperplasia / blood Prostatic Neoplasms / blood |
| Times Cited | 4 |
| WOS Category | BIOCHEMICAL RESEARCH METHODS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| Human and Animal Cells | |