RRC ID 45238
Author Phan HT, Vestergaard MC, Baek K, Shimokawa N, Takagi M.
Title Localization of amyloid beta (Aβ1-42) protofibrils in membrane lateral compartments: effect of cholesterol and 7-Ketocholesterol.
Journal FEBS Lett.
Abstract Cholesterol plays an important role in the interaction of Alzheimer's amyloid beta (Aβ) with cell membranes, an important event in Aβ-induced cytotoxicity. However, it is not fully understood how cholesterol influences the association of Aβ with membrane lateral compartments. We have shown that by modulating membrane fluidity, cholesterol decreased peptide localization in solid-ordered domains and increased that in liquid-ordered domains. It changed the amount of Aβ associating with liquid-disordered (Ld) phase with different tendencies depending on the composition of heterogeneous membrane systems. 7-Ketocholesterol, an oxidized derivative of cholesterol, majorly enhanced the fluidity of and Aβ interaction with Ld phase. These findings are useful for clarifying the impact of cholesterol and its oxidation in Aβ-induced toxicity.
Volume 588(18)
Pages 3483-90
Published 2014-9-17
DOI 10.1016/j.febslet.2014.08.007
PII S0014-5793(14)00608-5
PMID 25131929
MeSH Amyloid / metabolism* Amyloid beta-Peptides / metabolism* Cholesterol / physiology Humans Jurkat Cells Ketocholesterols / physiology* Liposomes / metabolism Membrane Fluidity Membrane Microdomains / metabolism Peptide Fragments / metabolism* Protein Transport
IF 2.675
Times Cited 3
WOS Category BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
Resource
Human and Animal Cells