論文 - 詳細
| RRC ID | 45258 |
|---|---|
| 著者 | Tani H, Onuma Y, Ito Y, Torimura M. |
| タイトル | Long non-coding RNAs as surrogate indicators for chemical stress responses in human-induced pluripotent stem cells. |
| ジャーナル | PLoS One |
| Abstract |
In this study, we focused on two biological products as ideal tools for toxicological assessment: long non-coding RNAs (lncRNAs) and human-induced pluripotent stem cells (hiPSCs). lncRNAs are an important class of pervasive non-protein-coding transcripts involved in the molecular mechanisms associated with responses to cellular stresses. hiPSCs possess the capabilities of self-renewal and differentiation into multiple cell types, and they are free of the ethical issues associated with human embryonic stem cells. Here, we identified six novel lncRNAs (CDKN2B-AS1, MIR22HG, GABPB1-AS1, FLJ33630, LINC00152, and LINC0541471_v2) that respond to model chemical stresses (cycloheximide, hydrogen peroxide, cadmium, or arsenic) in hiPSCs. Our results indicated that the lncRNAs responded to general and specific chemical stresses. Compared with typical mRNAs such as p53-related mRNAs, the lncRNAs highly and rapidly responded to chemical stresses. We propose that these lncRNAs have the potential to be surrogate indicators of chemical stress responses in hiPSCs. |
| 巻・号 | 9(8) |
| ページ | e106282 |
| 公開日 | 2014-1-1 |
| DOI | 10.1371/journal.pone.0106282 |
| PII | PONE-D-14-17808 |
| PMID | 25171338 |
| PMC | PMC4149554 |
| MeSH | Cell Line Gene Expression Regulation / drug effects* Humans Induced Pluripotent Stem Cells / cytology Induced Pluripotent Stem Cells / metabolism* RNA, Long Noncoding / biosynthesis* RNA, Messenger / biosynthesis Stress, Physiological / drug effects* Tumor Suppressor Protein p53 / biosynthesis |
| IF | 2.74 |
| 引用数 | 47 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 201B7(HPS0063) |