Reference - Detail
| RRC ID | 45261 |
|---|---|
| Author | Kuroda T, Yasuda S, Sato Y. |
| Title | In vitro detection of residual undifferentiated cells in retinal pigment epithelial cells derived from human induced pluripotent stem cells. |
| Journal | Methods Mol Biol |
| Abstract |
Human pluripotent stem cells (hPSCs) such as human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) are a leading candidate for regenerative medicine/cell therapies because of their capacity for pluripotency and unlimited self-renewal. However, there are significant obstacles preventing the clinical use of hPSCs. A significant safety issues is the presence of residual undifferentiated cells that have the potential to form tumors in vivo. Here, we describe the highly sensitive qRT-PCR methods for detection of residual undifferentiated cells in retinal pigment epithelial (RPE) cells derived from hiPSCs. qRT-PCR using probes and primers targeting LIN28A (LIN28) transcripts can detect residual undifferentiated cell levels as low as 0.002 % in hiPSC-derived RPE cells. We expect this method to contribute to process validation and quality control of hiPSC-derived cell therapy product. |
| Volume | 1210 |
| Pages | 183-92 |
| Published | 2014-1-1 |
| DOI | 10.1007/978-1-4939-1435-7_14 |
| PMID | 25173169 |
| MeSH | Cell Culture Techniques Cell Differentiation* / genetics Epithelial Cells / cytology* Epithelial Cells / metabolism Gene Expression Humans In Vitro Techniques Induced Pluripotent Stem Cells / cytology* Induced Pluripotent Stem Cells / metabolism Primary Cell Culture Retinal Pigment Epithelium / cytology* Retinal Pigment Epithelium / metabolism Reverse Transcriptase Polymerase Chain Reaction |
| Times Cited | 4 |
| WOS Category | BIOCHEMICAL RESEARCH METHODS CELL & TISSUE ENGINEERING BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Resource | |
| Human and Animal Cells | |