論文 - 詳細
| RRC ID | 45269 |
|---|---|
| 著者 | Kariya R, Matsuda K, Gotoh K, Vaeteewoottacharn K, Hattori S, Okada S. |
| タイトル | Establishment of nude mice with complete loss of lymphocytes and NK cells and application for in vivo bio-imaging. |
| ジャーナル | In Vivo |
| Abstract |
BACKGROUND:Nude mice are used in human xenograft research; however, only 25-35% of human tumors have been successfully transplanted into nude mice and their application is limited due to high natural killer (NK) cell activity. More severely immunodeficient mice with loss of NK activity are needed to overcome this limitation. MATERIALS AND METHODS:Balb/c nude Rag-2(-/-)Jak3(-/-) (Nude-RJ) mice were established by crossing Rag-2(-/-)Jak3(-/-) mice and nude mice. The K562 cell line was implanted subcutaneously to compare tumorigenicity between Nude-RJ mice and Nude mice. The cholangiocarcinoma mCherry expressing cell line (KKU-M213) was implanted subcutaneously, and fluorescence intensity and tumor weight were measured. RESULTS:Nude R/J mice showed complete loss of lymphocytes and NK cells. Xeno-transplantation of K562 cells showed higher proliferation in Nude R/J mice than nude mice. Subcutaneously-transplanted mCherry-transduced KKU-M213 cells were successfully detected with a fluorescence imager. CONCLUSION:Nude-R/J mice are valuable tools for in vivo imaging studies in biomedical research. |
| 巻・号 | 28(5) |
| ページ | 779-84 |
| 公開日 | 2014-1-1 |
| PII | 28/5/779 |
| PMID | 25189889 |
| MeSH | Animals Biomarkers Biopsy Cell Line, Tumor Disease Models, Animal Humans Killer Cells, Natural* / immunology Lymphocytes* / immunology Mice Mice, Knockout Mice, Nude Neoplasms / diagnosis* Neoplasms / immunology Neoplasms / pathology* Optical Imaging* / methods Severe Combined Immunodeficiency* Spleen / immunology Spleen / pathology Transplantation, Heterologous* Tumor Burden |
| IF | 1.541 |
| 引用数 | 7 |
| WOS 分野 | MEDICINE, RESEARCH & EXPERIMENTAL |
| リソース情報 | |
| ヒト・動物細胞 | K562 |