論文 - 詳細
| RRC ID | 45270 |
|---|---|
| 著者 | Garcia-Contreras R, Scougall-Vilchis RJ, Contreras-Bulnes R, Kanda Y, Nakajima H, Sakagami H. |
| タイトル | Effects of TiO2 nano glass ionomer cements against normal and cancer oral cells. |
| ジャーナル | In Vivo |
| Abstract |
BACKGROUND/AIM:Incorporation of nanoparticles (NPs) into the glass ionomer cements (GICs) is known to improve their mechanical and antibacterial properties. The present study aimed to investigate the possible cytotoxicity and pro-inflammation effect of three different powdered GICs (base, core build and restorative) prepared with and without titanium dioxide (TiO2) nanoparticles. MATERIALS AND METHODS:Each GIC was blended with TiO2 nanopowder, anatase phase, particle size <25 nm at 3% and 5% (w/w), and the GIC blocks of cements were prepared in a metal mold. The GICs/TiO2 nanoparticles cements were smashed up with a mortar and pestle to a fine powder, and then subjected to the sterilization by autoclaving. Human oral squamous cell carcinoma cell lines (HCS-2, HSC-3, HSC-4, Ca9-22) and human normal oral cells [gingival fibroblast (HGF), pulp (HPC) and periodontal ligament fibroblast (HPLF)] were incubated with different concentrations of GICs in the presence or absence of TiO2 nanoparticles, and the viable cell number was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method. Prostaglandin E2 was quantified by enzyme-linked immunosorbent assay (ELISA). Changes in fine cell structure were assessed by transmission electron microscopy. RESULTS:Cancer cells exhibited moderate cytotoxicity after 48 h of incubation, regardless of the type of GIC and the presence or absence of TiO2 NPs. GICs induced much lower cytotoxicity against normal cells, but induced prostaglandin E2 production, in a synergistic wanner with interleukin-1β. CONCLUSION:The present study shows acceptable to moderate biocompatibility of GICs impregnated with TiO2 nanoparticles, as well as its pro-inflammatory effects at higher concentrations. |
| 巻・号 | 28(5) |
| ページ | 895-907 |
| 公開日 | 2014-1-1 |
| PII | 28/5/895 |
| PMID | 25189906 |
| MeSH | Antineoplastic Agents / chemistry* Antineoplastic Agents / pharmacology* Antineoplastic Agents / toxicity Cell Line, Tumor Cell Survival / drug effects Dinoprostone / biosynthesis Dose-Response Relationship, Drug Glass Ionomer Cements / chemistry* Glass Ionomer Cements / pharmacology* Glass Ionomer Cements / toxicity Humans Mouth Neoplasms / metabolism Mouth Neoplasms / ultrastructure Nanoparticles / administration & dosage Nanoparticles / chemistry Titanium / administration & dosage* |
| IF | 1.541 |
| 引用数 | 17 |
| WOS 分野 | MEDICINE, RESEARCH & EXPERIMENTAL |
| リソース情報 | |
| ヒト・動物細胞 | Ca9-22(RCB1976) HSC-2(RCB1945) HSC-3(RCB1975) HSC-4(RCB1902) |