RRC ID |
45292
|
著者 |
Peraldo Neia C, Cavalloni G, Balsamo A, Venesio T, Napoli F, Sassi F, Martin V, Frattini M, Aglietta M, Leone F.
|
タイトル |
Screening for the FIG-ROS1 fusion in biliary tract carcinomas by nested PCR.
|
ジャーナル |
Genes Chromosomes Cancer
|
Abstract |
ROS1 rearrangements have been detected in a variety of tumors and are considered as suitable targets of anticancer therapies. We developed a new, quick, specific, and sensitive PCR test to screen for the FIG-ROS1 fusion and applied it to a series of Italian patients with bile duct carcinoma (BTC). Formalin-fixed, paraffin-embedded tissues, derived from 65 Italian BTC patients, and six cell lines were analyzed by nested PCR to investigate the prevalence of a previously reported FIG-ROS1 fusion. The specificity and sensitivity of nested PCR were investigated in FIG-ROS1 positive U118MG cells in reconstitution experiments with peripheral blood mononuclear cells. We found that six out of 65 (9%) BTC patients were positive for the FIG-ROS1 fusion, comprising two out of 14 (14%) gallbladder carcinoma (GBC) patients and four out of 25 (16%) extrahepatic cholangiocarcinoma (ECC) patients. None of the 26 intrahepatic cholangiocarcinoma cases harbored the FIG-ROS1 fusion. All the cell lines were negative for this variant. In conclusion, 14-16% of GBC and ECC were positive for FIG-ROS1. This may have clinical implications, since these patients will potentially benefit from the treatment with specific ROS1 inhibitors.
|
巻・号 |
53(12)
|
ページ |
1033-40
|
公開日 |
2014-12-1
|
DOI |
10.1002/gcc.22212
|
PMID |
25231053
|
MeSH |
Bile Duct Neoplasms / genetics*
Bile Duct Neoplasms / pathology
Carcinoma / pathology*
Cell Line, Tumor
Humans
Oncogene Proteins, Fusion / genetics*
Polymerase Chain Reaction
Sensitivity and Specificity
|
IF |
3.444
|
引用数 |
15
|
リソース情報 |
ヒト・動物細胞 |
HuH-28(RCB1943)
TGBC1TKB(RCB1129)
TFK-1(RCB2537) |