RRC ID 45304
Author Tawada M, Hayashi S, Ikegame Y, Nakashima S, Yoshida K.
Title Possible involvement of tumor-producing VEGF-A in the recruitment of lymphatic endothelial progenitor cells from bone marrow.
Journal Oncol Rep
Abstract Lymphatic metastasis of human malignant adenocarcinomas is a critical determinant of prognosis. Lymphangiogenesis, the growth of lymphatic vessels, is closely involved in lymphatic metastasis. However, the mechanisms of tumor lymphangiogenesis are not clearly understood. In a previous study, we showed that human gastric cancer MKN45 cells organize neighboring lymphatic vessels via recruitment of bone marrow-derived lymphatic endothelial progenitor cells in a nude mouse xenograft model. The present results also indicated that human colorectal cancer LS174T and breast cancer SK-BR-3 cells promoted lymphangiogenesis as well as the recruitment of lymphatic endothelial progenitor cells from bone marrow. Among growth factors, which are reported to be involved in lymphangiogenesis, only vascular endothelial growth factor (VEGF)-A was extensively secreted by these three types of adenocarcinoma cells in culture. The well-characterized lymphangiogenic factors VEGF-C and VEGF-D in the culture medium of these three types of adenocarcinoma cells were below the detectable levels in ELISA assay. Secretion of epidermal growth factor (EGF) and hepatocyte growth factor (HGF) was not detected. In in vitro culture assay, VEGF-A directly induced the differentiation of bone marrow mononuclear cells into LYVE-1-positive lymphatic endothelial lineage cells. These data collectively suggest the possibility that VEGF-A-rich human adenocarcinomas induce tumor lymphangiogenesis via recruitment of lymphangiogenic endothelial progenitor cells from bone marrow.
Volume 32(6)
Pages 2359-64
Published 2014-12-1
DOI 10.3892/or.2014.3499
PMID 25242215
MeSH Animals Bone Marrow / pathology* Bone Marrow Transplantation Cell Differentiation Cell Line, Tumor Cell Movement Endothelial Progenitor Cells / physiology* Humans Lymphatic Vessels / pathology* Mice, Inbred C57BL Mice, Nude Neoplasm Transplantation Vascular Endothelial Growth Factor A / metabolism*
IF 3.417
Times Cited 12
Human and Animal Cells